Soons P A, Kroon J M, Breimer D D
Division of Pharmacology, Leiden University, The Netherlands.
J Clin Pharmacol. 1990 Aug;30(8):693-8. doi: 10.1002/j.1552-4604.1990.tb03628.x.
The effects of single-dose (10 mg) and short-term (10 mg tid) nifedipine treatment on apparent hepatic blood flow, as assessed by indocyanine green (ICG) clearance, were studied in ten healthy male subjects. ICG was measured by both spectrophotometric and high performance liquid chromatography (HPLC) assay methods. Blood clearance of ICG and apparent hepatic blood flow were increased by 30 and 50%, respectively, after single-dose nifedipine, whereas after 4 days' treatment these values were 12 and 30%. The spectrophotometric assay significantly overestimated ICG plasma concentrations from 7 minutes onwards. Although the spectrophotometric and HPLC assay showed marked differences in calculated half-lives and volume of distribution of ICG, the ICG clearance values were similar for the two assay methods.
在10名健康男性受试者中研究了单剂量（10毫克）和短期（10毫克，每日三次）硝苯地平治疗对通过吲哚菁绿（ICG）清除率评估的表观肝血流量的影响。通过分光光度法和高效液相色谱（HPLC）测定方法测量ICG。单剂量硝苯地平后，ICG的血液清除率和表观肝血流量分别增加了30%和50%，而治疗4天后，这些值分别为12%和30%。从7分钟起，分光光度法显著高估了ICG血浆浓度。尽管分光光度法和HPLC测定法在计算的ICG半衰期和分布容积方面显示出明显差异，但两种测定方法的ICG清除率值相似。
