Sakamoto Yuhi, Terashita Nobuhiro, Muraguchi Takashi, Fukusato Toshio, Kubota Shunichiro
Department of Life Sciences, Graduate School of Arts and Sciences, The University of Tokyo.
Biosci Biotechnol Biochem. 2013;77(9):1799-803. doi: 10.1271/bbb.120882. Epub 2013 Sep 7.
Epigallocatechin 3-gallate (EGCG) has cytotoxic effects in many cancer cells. It has been reported that A549 lung cancer cells are markedly resistant to cell death induced by EGCG. In the present study, the effects of EGCG on A549 lung cancer cell growth and angiogenesis were studied. We found that EGCG dose-dependently suppressed A549 cell growth, while A549 cells were markedly resistant to cell death in vitro. Next we found that EGCG increased endostatin expression and suppressed vascular endothelial growth factor (VEGF) expression. We further studied to determine whether EGCG would suppress A549 tumor growth in nude mouse and angiogenesis. EGCG in drinking water significantly suppressed A549 tumor growth in nude mice. Histological analysis revealed that the number of CD34 positive vessels had a tendency to decrease in the tumor. In sum, EGCG had anti-proliferative effects of A549 on tumor growth and showed a tendency to suppress angiogenesis.
表没食子儿茶素-3-没食子酸酯（EGCG）对多种癌细胞具有细胞毒性作用。据报道，A549肺癌细胞对EGCG诱导的细胞死亡具有显著抗性。在本研究中，我们研究了EGCG对A549肺癌细胞生长和血管生成的影响。我们发现EGCG能剂量依赖性地抑制A549细胞生长，而A549细胞在体外对细胞死亡具有显著抗性。接下来我们发现EGCG能增加内皮抑素表达并抑制血管内皮生长因子（VEGF）表达。我们进一步研究以确定EGCG是否会抑制裸鼠体内A549肿瘤生长和血管生成。饮用水中的EGCG显著抑制了裸鼠体内A549肿瘤生长。组织学分析显示肿瘤中CD34阳性血管数量有减少趋势。总之，EGCG对A549肿瘤生长具有抗增殖作用，并显示出抑制血管生成的趋势。
