Kumar Suveen, Kumar Saurabh, Ali Md Azahar, Anand Pinki, Agrawal Ved Varun, John Renu, Maji Sagar, Malhotra Bansi D
Department of Biotechnology, Delhi Technological University, Shahbad Daulatpur, Delhi, India.
Biotechnol J. 2013 Nov;8(11):1267-79. doi: 10.1002/biot.201200386. Epub 2013 Sep 6.
There is a growing demand to integrate biosensors with microfluidics to provide miniaturized platforms with many favorable properties, such as reduced sample volume, decreased processing time, low cost analysis and low reagent consumption. These microfluidics-integrated biosensors would also have numerous advantages such as laminar flow, minimal handling of hazardous materials, multiple sample detection in parallel, portability and versatility in design. Microfluidics involves the science and technology of manipulation of fluids at the micro- to nano-liter level. It is predicted that combining biosensors with microfluidic chips will yield enhanced analytical capability, and widen the possibilities for applications in clinical diagnostics. The recent developments in microfluidics have helped researchers working in industries and educational institutes to adopt some of these platforms for point-of-care (POC) diagnostics. This review focuses on the latest advancements in the fields of microfluidic biosensing technologies, and on the challenges and possible solutions for translation of this technology for POC diagnostic applications. We also discuss the fabrication techniques required for developing microfluidic-integrated biosensors, recently reported biomarkers, and the prospects of POC diagnostics in the medical industry.
将生物传感器与微流体技术集成以提供具有许多优良特性的小型化平台的需求日益增长，这些特性包括减少样品体积、缩短处理时间、低成本分析和低试剂消耗。这些集成了微流体技术的生物传感器还具有诸多优势，如层流、对危险材料的处理最少、可并行进行多个样品检测、便携性以及设计的通用性。微流体技术涉及在微升至纳升水平操控流体的科学与技术。据预测，将生物传感器与微流体芯片相结合将提升分析能力，并拓宽其在临床诊断中的应用可能性。微流体技术的最新进展已帮助工业界和教育机构的研究人员采用其中一些平台用于即时检测（POC）诊断。本综述聚焦于微流体生物传感技术领域的最新进展，以及将该技术转化用于POC诊断应用所面临的挑战和可能的解决方案。我们还讨论了开发集成微流体的生物传感器所需的制造技术、最近报道的生物标志物以及POC诊断在医疗行业的前景。
