Nan Ma, Yangmei Chen, Bangcheng Yang
Engineering Research Center in Biomaterials, Sichuan University, Chengdu, 610064, China; National Engineering Research Center for Biomaterials, Chengdu, 610064, China.
J Biomed Mater Res A. 2014 Aug;102(8):2644-51. doi: 10.1002/jbm.a.34933. Epub 2013 Sep 10.
Reactive oxygen plays an important role in the pathogenesis of many serious illnesses, including bony cancer. Recently, it has been suggested that hydrogen (H2), a selective antioxidant, can effectively scavenge free radicals. Biomedical magnesium (Mg) used for bone defect repair in the surgery of bony cancer could release H2 because of the degradation, so Mg might have the potential to prevent bony cancer from metastasis and recurrence. In this study, alkali-heat treatment method was employed to modify the surface structure of Mg metal, so as to control the degradation of Mg metal and the H2 releasing rate. Then the released H2 was introduced to the Fenton Reaction system to detect its effect on scavenging free radicals. The modified Mg metal was employed as the substrate for bone cancer cell culture to study the effect of the H2 releasing on scavenging free radicals in the cells. It is found that the H2 released from the Mg degradation could scavenge free radicals both in the Fenton Reaction system and bone cancer cells. The effect on the scavenging free radical is proportional to the rate of H2 releasing. It suggested that Mg might be a potential material with anti-bone cancer properties. It is hopeful to both repair the bone defect and prevent bony cancer from metastasis and recurrence for the bony cancer patients by biomedical Mg metal.
活性氧在包括骨癌在内的许多严重疾病的发病机制中起着重要作用。最近，有人提出，作为一种选择性抗氧化剂的氢气（H₂）可以有效清除自由基。用于骨癌手术中骨缺损修复的生物医学镁（Mg）由于降解会释放H₂，因此Mg可能具有预防骨癌转移和复发的潜力。在本研究中，采用碱热处理方法来改变Mg金属的表面结构，以控制Mg金属的降解和H₂释放速率。然后将释放的H₂引入芬顿反应体系中，检测其对清除自由基的效果。将改性后的Mg金属用作骨癌细胞培养的底物，以研究H₂释放对清除细胞内自由基的影响。研究发现，Mg降解释放的H₂既能在芬顿反应体系中清除自由基，也能在骨癌细胞中清除自由基。对清除自由基的效果与H₂释放速率成正比。这表明Mg可能是一种具有抗骨癌特性的潜在材料。有望通过生物医学Mg金属为骨癌患者修复骨缺损并预防骨癌转移和复发。
