Szymanska H, Lechowska-Piskorowska J, Krysiak E, Strzalkowska A, Unrug-Bielawska K, Grygalewicz B, Skurzak H M, Pienkowska-Grela B, Gajewska M
Maria Sklodowska-Curie Memorial Cancer Center and Institute of Oncology, Roentgena 5, Warsaw 02-781, Poland. Email:
Vet Pathol. 2014 May;51(3):663-79. doi: 10.1177/0300985813501334. Epub 2013 Sep 9.
The evaluation of spontaneous lesions in classical inbred strains of mice has become increasingly important because genetically engineered mice (GEMs) are created on these backgrounds. Novel inbred strains-genetically diverse from classic strains-are valuable both as a new background for GEM mice and to increase the genetic variation found in laboratory mice. Newly arising spontaneous genetic alterations in commonly used strains may also lead to new and valuable mouse models of disease. This report evaluates gross and histological lesions in relatively new, classic, and rarely explored mouse inbred strains. Pathological lesions of 1273 mice from 12 inbred strains (129S1/SvW, A.CA-H2(f) /W, AKR/W, BALB/cW, BN/aW, C57BL/6 W, C57BL/10 W, C3H/W, C3H (wad) /W, CBA/W, DBA/2 W, and WOM/W) are reported. BN/aW, WOM/W, and C3H (wad) /W are novel inbred strains produced and maintained in the Department of Genetics and Laboratory Animal Breeding at the Center of Oncology, Warsaw, Poland. Both neoplastic and nonneoplastic lesions were examined. The prevalence of lung neoplasms was significantly higher in A.CA-H2(f) /W (33.3%) and BALB/cW (33.8%) mice (P < .01). The prevalence of liver neoplasms was significantly higher in the CBA/W strain (P < .01). Mammary gland neoplasms arose at a greater frequency in C3H/W mice (P < .01). The occurrence of uterine neoplasms was higher in DBA/W and 129S1/SvW mice. AKR/W and WOM/W mice developed T-cell lymphoblastic lymphoma with high frequency (110/121 [90.9%] and 159/175 [90.9%], respectively) before 1 year of age. The occurrence of nonneoplastic lesions in the kidneys of BN/aW mice was increased (P < .01).
对经典近交系小鼠自发病变的评估变得越来越重要，因为基因工程小鼠（GEMs）是在这些背景品系上培育出来的。与经典品系基因不同的新型近交系，作为GEM小鼠的新背景以及增加实验室小鼠的遗传多样性都很有价值。常用品系中新出现的自发基因改变也可能产生新的、有价值的疾病小鼠模型。本报告评估了相对较新的、经典的以及很少被研究的小鼠近交系中的大体和组织学病变。报告了来自12个近交系（129S1/SvW、A.CA-H2(f)/W、AKR/W、BALB/cW、BN/aW、C57BL/6 W、C57BL/10 W、C3H/W、C3H (wad)/W、CBA/W、DBA/2 W和WOM/W）的1273只小鼠的病理病变。BN/aW、WOM/W和C3H (wad)/W是在波兰华沙肿瘤学中心遗传学与实验动物育种部培育和维持的新型近交系。对肿瘤性和非肿瘤性病变均进行了检查。A.CA-H2(f)/W（33.3%）和BALB/cW（33.8%）小鼠的肺肿瘤发生率显著更高（P <.01）。CBA/W品系的肝肿瘤发生率显著更高（P <.01）。C3H/W小鼠乳腺肿瘤的发生频率更高（P <.01）。DBA/W和129S1/SvW小鼠子宫肿瘤的发生率更高。AKR/W和WOM/W小鼠在1岁前高频发生T细胞淋巴母细胞淋巴瘤（分别为110/121 [90.9%]和159/175 [90.9%]）。BN/aW小鼠肾脏中非肿瘤性病变的发生率增加（P <.01）。
