TLR 激动剂共价修饰细胞表面可改善并指导免疫刺激。
Covalent modification of cell surfaces with TLR agonists improves & directs immune stimulation.
机构信息
Department of Chemistry, University of California Irvine, Irvine, CA 92697, USA.
出版信息
Chem Commun (Camb). 2013 Oct 25;49(83):9618-20. doi: 10.1039/c3cc45468a.
Abstract
We present a primary example of a cell surface modified with a synergistic combination of agonists to tune immune stimulation. A model cell line, Lewis Lung Carcinoma, was covalently modified with CpG-oligonucleotides and lipoteichoic acid, both Toll-like receptor (TLR) agonists. The immune-stimulating constructs provided greater stimulation of NF-κB in a model cell line and bone marrow-derived dendritic cells than the components unconjugated in solution.
摘要
我们提出了一个细胞表面经过协同组合激动剂修饰的主要例子，以调节免疫刺激。使用 CpG 寡核苷酸和脂磷壁酸（均为 Toll 样受体 (TLR) 激动剂）对 Lewis 肺癌细胞系进行了共价修饰。与游离溶液中的成分相比，这些免疫刺激构建体在模型细胞系和骨髓来源的树突状细胞中提供了更强的 NF-κB 刺激。
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Adv Funct Mater. 2013 Jan 14;23(2):161-172. doi: 10.1002/adfm.201201512.
2
A high-throughput nanoimmunoassay chip applied to large-scale vaccine adjuvant screening.高通量纳米免疫分析芯片在大规模疫苗佐剂筛选中的应用。
Integr Biol (Camb). 2013 Apr;5(4):650-8. doi: 10.1039/c3ib20263a. Epub 2013 Feb 26.
3
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5
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