Dodd I B, Egan J B
Department of Biochemistry, University of Adelaide, Australia.
Nucleic Acids Res. 1990 Sep 11;18(17):5019-26. doi: 10.1093/nar/18.17.5019.
We present an update of our method for systematic detection and evaluation of potential helix-turn-helix DNA-binding motifs in protein sequences [Dodd, I. and Egan, J. B. (1987) J. Mol. Biol. 194, 557-564]. The new method is considerably more powerful, detecting approximately 50% more likely helix-turn-helix sequences without an increase in false predictions. This improvement is due almost entirely to the use of a much larger reference set of 91 presumed helix-turn-helix sequences. The scoring matrix derived from this reference set has been calibrated against a large protein sequence database so that the score obtained by a sequence can be used to give a practical estimation of the probability that the sequence is a helix-turn-helix motif.
我们展示了我们用于系统检测和评估蛋白质序列中潜在螺旋-转角-螺旋DNA结合基序的方法的更新内容[多德，I.和伊根，J.B.（1987年）《分子生物学杂志》194卷，557 - 564页]。新方法的功能强大得多，能检测出可能性高出约50%的螺旋-转角-螺旋序列，且不会增加错误预测。这种改进几乎完全归功于使用了一个大得多的由91个假定的螺旋-转角-螺旋序列组成的参考集。从该参考集得出的评分矩阵已针对一个大型蛋白质序列数据库进行了校准，这样一个序列获得的分数可用于实际估计该序列是螺旋-转角-螺旋基序的概率。
