Dube Syamalima, Saksena Nitin, Spicer Timothy, Healey Jayne, Benz Patricia, Dube Dipak K, Poiesz Bernard J
Division of Hematology/Oncology, Department of Medicine, State University of New York, Upstate Medical University, 750 East Adams Street, Syracuse, NY 13210, USA.
Virol J. 2013 Sep 11;10:282. doi: 10.1186/1743-422X-10-282.
Simian T-cell lymphoma/leukemia virus-1 (STLV-1) infection of non-human primates can serve as a model for human T-cell lymphoma/leukemia virus infection.
Two tantalus and 2 patas monkeys were transfused with intraspecies whole blood infected with STLV-1. Infection was determined by ELISA, western blot and DNA PCR analyses. The entire genome of the STLV-1 Tan 90 strain and some of the STVL-1 Pat74 strain were amplified using over-lapping primer-pairs and subsequently sequenced.
Followup studies conducted over 2 years indicated that all 4 monkeys remained healthy despite being infected with STLV-1, as determined by PCR, cloning and sequencing analyses. ELISA and Western blot analyses indicated that both patas monkeys seroconverted within 2 months of transfusion, while one tantalus monkey required one year to seroconvert and the other never fully seroconverted. The tantalus monkey which never fully seroconverted, failed to react to HTLV-1 p24 Gag antigen. Sequence analyses indicated that, while unique, the deduced p24 Gag amino acid sequence of the STLV-1 Tan 90 strain used for infection was still highly homologous to the HTLV-1 p24 Gag amino acids present in the ELISA and WB assays. However, a mutation in the pol sequence of STLV-1 Tan 90 encoded a putative stop codon, while a common deletion in the pol/rex regulatory gene causes significant changes in the Pol, and p27 Rex proteins. These same mutations were also observed in the viral DNA of both recipient infected tantalus monkeys and were not present in the STLV-1 Pat 74 strain.
Our data suggest that seroconversion to STLV-1 infection may be prolonged due to the above mutations, and that compensatory molecular events must have occurred to allow for virus transmission.
非人灵长类动物感染猿猴T细胞淋巴瘤/白血病病毒1型（STLV-1）可作为人类T细胞淋巴瘤/白血病病毒感染的模型。
给2只草原狒狒和2只赤猴输注感染STLV-1的同种全血。通过酶联免疫吸附测定（ELISA）、蛋白质免疫印迹法和DNA聚合酶链式反应（PCR）分析确定感染情况。使用重叠引物对扩增STLV-1 Tan 90株的全基因组和部分STVL-1 Pat74株的基因组，随后进行测序。
超过2年的随访研究表明，通过PCR、克隆和测序分析确定，所有4只猴子尽管感染了STLV-1，但仍保持健康。ELISA和蛋白质免疫印迹分析表明，2只赤猴在输血后2个月内血清阳转，而1只草原狒狒需要1年才血清阳转，另1只从未完全血清阳转。从未完全血清阳转的草原狒狒对人嗜T淋巴细胞病毒1型（HTLV-1）p24 Gag抗原无反应。序列分析表明，用于感染的STLV-1 Tan 90株推导的p24 Gag氨基酸序列虽然独特，但与ELISA和蛋白质免疫印迹检测中存在的HTLV-1 p24 Gag氨基酸仍高度同源。然而，STLV-1 Tan 90株pol序列中的一个突变编码了一个推定的终止密码子，而pol/rex调控基因中的一个常见缺失导致Pol和p27 Rex蛋白发生显著变化。在受感染的草原狒狒受体的病毒DNA中也观察到相同的突变，而STLV-1 Pat 74株中不存在这些突变。
我们的数据表明，由于上述突变，STLV-1感染的血清阳转可能会延长，并且必定发生了补偿性分子事件以实现病毒传播。
