Simkin Jennifer, Han Manjong, Yu Ling, Yan Mingquan, Muneoka Ken
Division of Cell and Molecular Biology, Tulane University, New Orleans, LA, USA.
Methods Mol Biol. 2013;1037:419-35. doi: 10.1007/978-1-62703-505-7_24.
A challenge to the study of regeneration is determining at what point the processes of wound healing and regeneration diverge. The mouse displays level-specific regeneration responses. An amputation through the distal third of the terminal phalanx will prompt a regeneration response and result in a new digit tip that mimics the morphology of the lost digit tip. Conversely, an amputation through the distal third of the intermediate phalanx initiates a wound healing and scarring response. The mouse, therefore, provides a model for studying the transition between wound healing and regeneration in the same animal. This chapter details the methods used in the study of mammalian digit regeneration, including a method to introduce exogenous protein into the mouse digit amputation model via microcarrier beads and methods for analysis of bone regeneration.
再生研究面临的一个挑战是确定伤口愈合和再生过程在何时出现分歧。小鼠表现出特定水平的再生反应。通过末节指骨远端三分之一处进行截肢会引发再生反应，并产生一个新的指尖，其形态与失去的指尖相似。相反，通过中节指骨远端三分之一处进行截肢则会引发伤口愈合和瘢痕形成反应。因此，小鼠为研究同一动物体内伤口愈合和再生之间的转变提供了一个模型。本章详细介绍了在哺乳动物指再生研究中使用的方法，包括通过微载体珠将外源蛋白引入小鼠指截肢模型的方法以及骨再生分析方法。
