Dept of Molecular Biology and Biotechnology, University of Sheffield, Firth Court, Western Bank, Sheffield S10 2TN, UK.
Chem Commun (Camb). 2013 Oct 28;49(84):9824-6. doi: 10.1039/c3cc45602a.
We show that salt bridges involving lysines can be detected by deuterium isotope effects on NMR chemical shifts of the sidechain amine. Lys27 in the ribonuclease barnase is salt bridged, and mutation of Arg69 to Lys retains a partially buried salt bridge. The salt bridges are functionally important.
我们证明，通过侧链胺的 NMR 化学位移的氘同位素效应，可以检测涉及赖氨酸的盐桥。核糖核酸酶 barnase 中的 Lys27 形成盐桥，将 Arg69 突变为 Lys 保留了部分埋藏的盐桥。盐桥在功能上很重要。
