一种使用经过合理设计的配体-RNA 对进行操作的合成核糖开关。
A synthetic riboswitch that operates using a rationally designed ligand-RNA pair.
机构信息
The Institute of Scientific and Industrial Research, Osaka University, 8-1 Mihogaoka, Ibaraki, Osaka 567-0047 (Japan); PRESTO (Japan) Science and Technology Agency, 4-1-8 Honcho Kawaguchi, Saitama 332-0012 (Japan).
出版信息
Angew Chem Int Ed Engl. 2013 Sep 16;52(38):9976-9. doi: 10.1002/anie.201303370. Epub 2013 Sep 3.
The construction of an artificial riboswitch is based on a ligand-RNA pair without any molecular biology-based selection processes. The ligand selectively and significantly stabilized an RNA duplex containing an r(XGG)/r(XGG) sequence (X=U, A, G). The integration of the ligand-binding sequences into the 5'-untranslated region of mRNA provided an artificial riboswitch that was responsive to Z-NCTS.
人工核糖开关的构建基于配体-RNA 对，而不涉及任何基于分子生物学的选择过程。该配体选择性地并显著稳定了含有 r(XGG)/r(XGG)序列（X=U、A、G）的 RNA 双链体。将配体结合序列整合到 mRNA 的 5'-非翻译区中，提供了一种对 Z-NCTS 有响应的人工核糖开关。
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