Departamento de Microbiologia e Imunologia, Instituto de Biociências, Universidade Estadual Paulista , Botucatu, SP , Brasil.
Braz J Microbiol. 2010 Jan;41(1):59-65. doi: 10.1590/S1517-838220100001000011. Epub 2010 Mar 1.
Food handlers, an important factor in food quality, may contain bacteria that are able to cause foodborne disease. The present study aimed to research coagulase-negative (CNS) and -positive staphylococci (CPS) in 82 food handlers, analyzing nasal and hand swabs, with identification of 62 CNS (75.6%) and 20 CPS strains (24.4%). Staphylococcal enterotoxins genes were investigated by PCR. In 20 CPS strains, 19 were positive for one or more genes. The percentage of CNS presenting genes for enterotoxins was high (46.8%). Despite of the staphylococcal species, the most common gene was sea (35.4%), followed by seh and sej (29.2%). The detection of new staphylococcal enterotoxins (SEs) genes showed a higher pathogenic potential in this genus. The presence of these gene points out the importance of CNS not only as contaminant bacteria but also as a pathogen.
食品从业人员是食品质量的一个重要因素，他们可能携带能够引起食源性疾病的细菌。本研究旨在研究 82 名食品从业人员的凝固酶阴性（CNS）和阳性葡萄球菌（CPS），分析鼻拭子和手拭子，鉴定出 62 株 CNS（75.6%）和 20 株 CPS 菌株（24.4%）。采用 PCR 方法检测葡萄球菌肠毒素基因。在 20 株 CPS 菌株中，有 19 株携带一种或多种基因。携带肠毒素基因的 CNS 百分比较高（46.8%）。不管是哪种葡萄球菌，最常见的基因是 sea（35.4%），其次是seh 和 sej（29.2%）。新型葡萄球菌肠毒素（SEs）基因的检测表明该属具有更高的致病性潜力。这些基因的存在不仅表明 CNS 是污染菌，而且是一种病原体。
