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基于 16S rRNA 基因的 PCR-变性梯度凝胶电泳（PCR-DGGE）指纹图谱分析鉴定术后眼内炎中的细菌。

16S rRNA gene-based identification of bacteria in postoperative endophthalmitis by PCR-Denaturing Gradient Gel Electrophoresis (PCR-DGGE) fingerprinting.

机构信息

Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas-IPN , Mexico, D.F. Mexico.

出版信息

Braz J Microbiol. 2012 Jan;43(1):283-7. doi: 10.1590/S1517-838220120001000033. Epub 2012 Jun 1.

DOI:10.1590/S1517-838220120001000033
PMID:24031830
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3768976/
Abstract

Conventional microbiological culture techniques are frequently insufficient to confirm endophthalmitis clinical cases which could require urgent medical attention because it could lead to permanent vision loss. We are proposing PCR-DGGE and 16S rRNA gene libraries as an alternative to improve the detection and identification rate of bacterial species from endophthalmitis cases.

摘要

传统的微生物培养技术常常不足以确认需要紧急医疗关注的眼内炎临床病例，因为这可能导致永久性视力丧失。我们建议使用 PCR-DGGE 和 16S rRNA 基因文库作为替代方法，以提高眼内炎病例中细菌物种的检测和鉴定率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b764/3768976/4b8368b54e1d/bjm-43-283-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b764/3768976/4b8368b54e1d/bjm-43-283-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/b764/3768976/4b8368b54e1d/bjm-43-283-g001.jpg

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本文引用的文献

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