Laboratory of Applied Microbiology and Biotechnology, Department of Molecular Biology & Genetics, Democritus University of Thrace, 681 00, Alexandroupolis, Greece.
Environ Sci Pollut Res Int. 2018 Mar;25(8):7401-7408. doi: 10.1007/s11356-017-1051-6. Epub 2017 Dec 26.
An alcoholic fermentation process is described, involving molasses, the main by-product of the sugar industry, blended with crude olive oil mill wastewaters (OOMWs) and immobilized Saccharomyces cerevisiae cells on delignified cellulosic material (DCM). For comparison, fermentations with free cells were also carried out. Initially, the optimum blending mixture for molasses dilution was sought after, while at a second step repeated batch fermentations at a temperature range 5-30 °C were performed to monitor the operational stability of the system. A 1/1 ratio of OOMWs/tap water blending mixture and cell immobilization resulted in higher fermentation parameters. Ethanol concentration and daily productivity values recorded at temperatures ≥ 20 °C (up to 67.8 g L and 67.6 g L d, respectively) could be adopted by the industrial sector, although the decline in fermentation efficiency observed, probably due to the toxicity effects of OOMWs. Finally, the potential of OOMWs treatment for ethanol production is highlighted and assessed.
介绍了一种酒精发酵工艺，该工艺涉及糖工业的主要副产物——糖蜜，与粗橄榄油厂废水（OOMWs）和固定化酿酒酵母细胞在去木质纤维素材料（DCM）上混合。为了进行比较，还进行了游离细胞发酵。最初，在寻求最佳糖蜜稀释混合比之后，在 5-30°C 的温度范围内进行重复分批发酵，以监测系统的操作稳定性。OOMWs/自来水混合比为 1/1 和细胞固定化导致了更高的发酵参数。在温度≥20°C 时，记录到的乙醇浓度和日产量值（分别高达 67.8 g/L 和 67.6 g/L·d）可被工业部门采用，尽管观察到发酵效率下降，这可能是由于 OOMWs 的毒性作用所致。最后，强调并评估了 OOMWs 处理用于生产乙醇的潜力。
