Jackson M A, Bennett G A
Fermentation Biochemistry Research, U.S. Department of Agriculture, Peoria, Illinois 61604.
Appl Environ Microbiol. 1990 Aug;56(8):2296-8. doi: 10.1128/aem.56.8.2296-2298.1990.
Fumonisin B1, a recently discovered mycotoxin, was synthesized by submerged cultures of Fusarium moniliforme NRRL 13616 grown for 29 days at 28 degrees C and 220 rpm in a basal salts medium (pH 5.0) supplemented with 90 g of glucose per liter and 3.5 g of ammonium sulfate per liter. Under these culture conditions, 74 +/- 23 micrograms of fumonisin B1 per ml was produced by 29-day-old F. moniliforme NRRL 13616 cultures. Fumonisin B1 was detected in liquid culture extracts by high-performance thin-layer chromatography. Fumonisin B1 was confirmed and quantitated by gas chromatography and gas chromatography-mass spectral analysis of the trimethylsilyl derivative. The use of a defined medium for producing fumonisin B1 in a submerged culture facilitates its isolation and provides an excellent method for conducting biosynthetic studies.
伏马菌素B1是一种最近发现的霉菌毒素，由串珠镰刀菌NRRL 13616在基础盐培养基（pH 5.0）中于28℃、220转/分钟的条件下进行29天的深层培养合成，该培养基每升补充90克葡萄糖和3.5克硫酸铵。在这些培养条件下，29日龄的串珠镰刀菌NRRL 13616培养物每毫升产生74±23微克伏马菌素B1。通过高效薄层色谱法在液体培养提取物中检测到伏马菌素B1。通过气相色谱法以及对三甲基硅烷基衍生物的气相色谱 - 质谱分析对伏马菌素B1进行确认和定量。在深层培养中使用特定培养基生产伏马菌素B1有助于其分离，并为进行生物合成研究提供了一种出色的方法。
