Branham B E, Plattner R D
Michigan State University, Department of Crop and Soil Sciences.
Mycopathologia. 1993 Nov;124(2):99-104. doi: 10.1007/BF01103109.
The biosynthesis of fumonisin B1 by Fusarium moniliforme was studied in liquid culture. Stable isotope labeled alanine was added as a precursor to static and shaken cultures of F. moniliforme. Incorporation of 13C and 2H labeled L-alanine into fumonisin B1 was measured by GC-MS. Under static culture conditions, 20.8 micrograms ml-1 of FB1 were produced with a 5.5% level of incorporation of intact, labeled alanine into fumonisin B1. Under shake culture conditions, much higher levels of fumonisin B1 were produced with levels reaching 159-240 micrograms ml-1 by 21 days after culture initiation. A lower level of alanine incorporation, from 1.1-1.4%, was observed under these conditions. Under shake conditions, incorporation of labeled alanine was reduced because of the rapid metabolism of these cultures combined with the high level of fumonisin B1 production resulted in rapid turnover of the added, labeled alanine and reduced percentage of incorporation. The evidence presented indicates that alanine is incorporated intact into fumonisin B1.
在液体培养中研究了串珠镰刀菌产生伏马菌素B1的生物合成过程。将稳定同位素标记的丙氨酸作为前体添加到串珠镰刀菌的静置培养物和振荡培养物中。通过气相色谱 - 质谱法测定13C和2H标记的L - 丙氨酸掺入伏马菌素B1中的情况。在静置培养条件下，产生了20.8微克/毫升的FB1，完整的标记丙氨酸掺入伏马菌素B1的水平为5.5%。在振荡培养条件下，伏马菌素B1的产量要高得多，培养开始21天后达到159 - 240微克/毫升。在这些条件下观察到较低的丙氨酸掺入水平，为1.1 - 1.4%。在振荡条件下，标记丙氨酸的掺入减少，这是因为这些培养物的快速代谢以及伏马菌素B1的高产量导致添加的标记丙氨酸快速周转，掺入百分比降低。所提供的证据表明丙氨酸完整地掺入了伏马菌素B1中。
