RNA纯化——沉淀方法。
RNA purification--precipitation methods.
作者信息
Walker Sarah E, Lorsch Jon
机构信息
Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD, USA.
出版信息
Methods Enzymol. 2013;530:337-43. doi: 10.1016/B978-0-12-420037-1.00019-1.
Abstract
When working with RNA, the need often arises to concentrate a sample or purify it from various salts, nucleotides, and proteins. RNA precipitation is an easy and cost-effective method for the concentration of RNA, leaving a pellet that can be resuspended in the buffer of choice.
摘要
在处理RNA时，常常需要浓缩样品或从各种盐、核苷酸和蛋白质中纯化它。RNA沉淀是一种简单且经济高效的RNA浓缩方法，会留下一个沉淀，该沉淀可重悬于所需的缓冲液中。
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