Laboratory for Experimental Haematology, Immunology and Oncology, Division of Molecular Medicine, Rudjer Boskovic Institute, Bijenicka c. 54, HR-10000 Zagreb, Croatia.
Neuropeptides. 2013 Oct;47(5):315-9. doi: 10.1016/j.npep.2013.08.002. Epub 2013 Aug 28.
Due to their involvement in dependence pathways, opioid system genes represent strong candidates for association studies investigating alcoholism. In this study, single nucleotide polymorphisms within the genes for mu (OPRM1) and kappa (OPRK1) opioid receptors and precursors of their ligands - proopiomelanocortin (POMC), coding for beta-endorphin and prodynorphin (PDYN) coding for dynorphins, were analyzed in a case-control study that included 354 male alcohol-dependent and 357 male control subjects from Croatian population. Analysis of allele and genotype frequencies of the selected polymorphisms of the genes OPRM1/POMC and OPRK1/PDYN revealed no differences between the tested groups. The same was true when alcohol-dependent persons were subdivided according to the Cloninger's criteria into type-1 and type-2 groups, known to differ in the extent of genetic control. Thus, the data obtained suggest no association of the selected polymorphisms of the genes OPRM1/POMC and OPRK1/PDYN with alcoholism in Croatian population.
由于它们参与了依赖途径，阿片系统基因代表了与酒精中毒相关的关联研究的强有力候选者。在这项研究中，对来自克罗地亚人群的 354 名男性酒精依赖者和 357 名男性对照者进行了病例对照研究，分析了阿片受体和其配体前体 - 促黑皮质素原（POMC）的基因（OPRM1）和κ（OPRK1）阿片受体以及编码β-内啡肽和强啡肽（PDYN）的基因中的单核苷酸多态性。对选定的 OPRM1/POMC 和 OPRK1/PDYN 基因多态性的等位基因和基因型频率分析表明，测试组之间没有差异。当根据克隆纳的标准将酒精依赖者分为已知在遗传控制程度上不同的 1 型和 2 型组时，情况也是如此。因此，获得的数据表明，在克罗地亚人群中，选定的 OPRM1/POMC 和 OPRK1/PDYN 基因多态性与酒精中毒无关。
