Nisa Sobia, Bibi Yamin, Zia Muhammad, Waheed Abdul, Chaudhary M Fayyaz
Department of Microbiology, University of Haripur, KPK, Pakistan.
Pak J Pharm Sci. 2013 Sep;26(5):1009-12.
This study was aimed to determine the effectiveness of two ethnobotanically important plant species Carissa opaca and Toona ciliata against cancer cells. Antiproliferative activity of the plant extracts and their fractions was tested against MCF-7 breast cancer cell line using MTT assay. A concentration dependent inhibition was observed for both crude extracts. C. opaca crude extract showed 78.5% inhibition while T. ciliata showed 57% activity against cancer cells at 500 μg/mL. Fractions were tested at 200 μg/mL concentration and were more active than crude extracts. Chloroform fraction of C. opaca showed maximum inhibition 99% followed by ethyl acetate and methanol fraction of C. opaca exhibiting 96% and 94% inhibition, respectively. Ethyl acetate fraction of T. ciliata showed 78% inhibition of cancer cells at the same concentration. Preliminary phytochemical screening revealed the chemical composition of C. opaca extract containing alkaloids, flavonoids, tannins and saponins while T. ciliata had tannins and coumarins. Present investigation suggests that tested plant species possess potent anticancer compounds specially chloroform, ethyl acetate and methanol fractions of C. opaca and ethyl acetate fraction of T. ciliata can be an important source of anticancer drugs.
本研究旨在确定两种具有重要民族植物学意义的植物物种——暗罗和红椿对癌细胞的有效性。使用MTT法检测了植物提取物及其馏分对MCF-7乳腺癌细胞系的抗增殖活性。两种粗提物均观察到浓度依赖性抑制作用。在500μg/mL时，暗罗粗提物对癌细胞的抑制率为78.5%，而红椿的抑制活性为57%。对馏分在200μg/mL浓度下进行了测试，其活性比粗提物更高。暗罗的氯仿馏分显示出最大抑制率99%，其次是暗罗的乙酸乙酯馏分和甲醇馏分，分别表现出96%和94%的抑制率。在相同浓度下，红椿的乙酸乙酯馏分对癌细胞的抑制率为78%。初步植物化学筛选显示，暗罗提取物的化学成分含有生物碱、黄酮类、单宁和皂苷，而红椿含有单宁和香豆素。目前的研究表明，受试植物物种含有有效的抗癌化合物，特别是暗罗的氯仿、乙酸乙酯和甲醇馏分以及红椿的乙酸乙酯馏分可能是抗癌药物的重要来源。
