Department of Immunology, Tehran University of Medical Sciences, Tehran, Iran.
Microb Pathog. 2013 Dec;65:7-13. doi: 10.1016/j.micpath.2013.08.005. Epub 2013 Sep 10.
Helicobacter pylori (Hp) infection is associated with gastritis and marked infiltration of the gastric mucosa by several cytokines secreting inflammatory cells that contribute to sustain and expand the local inflammation. Different clinical expressions of the infection may reflect distinctive patterns of cytokine expression. IL-1β, TNF-α, IL-17 and IL-23 have been reported to be involved in Hp-induced gastric mucosal inflammation, but the details and association to different patterns of inflammation and virulence factors remain unclear.
Total RNA was extracted from gastric biopsies of 51 Hp-infected patients and 44 Hp-negative patients. Mucosal IL-18 mRNA expression in gastric biopsies was determined by Real-Time PCR. Presence of virulence factors was evaluated using PCR.
IL-18 mRNA expression was significantly increased in biopsies of Hp-infected patients compared to Hp-uninfected individuals. There was no association between virulence factors and IL-18 mRNA expression. Also severity of mononuclear infiltration was significantly higher in gastritis patients with vacA (m1)-positive compare patients with vacA (m2)-positive.
IL-18 may play an important role in the inflammatory response and promote the chronic and persistent inflammatory changes in the stomach. This may ultimately influence the outcome of Hp-associated diseases that arise within the context of gastritis.
幽门螺杆菌（Hp）感染与胃炎有关，几种分泌炎症细胞的细胞因子显著浸润胃黏膜，有助于维持和扩大局部炎症。感染的不同临床表现可能反映了细胞因子表达的不同模式。IL-1β、TNF-α、IL-17 和 IL-23 已被报道参与 Hp 诱导的胃黏膜炎症，但细节以及与不同炎症模式和毒力因子的关联仍不清楚。
从 51 例 Hp 感染患者和 44 例 Hp 阴性患者的胃活检组织中提取总 RNA。采用 Real-Time PCR 检测胃活检组织中黏膜 IL-18 mRNA 的表达。采用 PCR 评估毒力因子的存在。
与 Hp 未感染个体相比，Hp 感染患者的活检组织中 IL-18 mRNA 表达显著增加。毒力因子与 IL-18 mRNA 表达之间没有关联。此外，在 vacA（m1）阳性的胃炎患者中，单核细胞浸润的严重程度明显高于 vacA（m2）阳性的患者。
IL-18 可能在炎症反应中发挥重要作用，并促进胃内慢性和持续的炎症变化。这可能最终影响在胃炎背景下发生的与 Hp 相关疾病的结局。
