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大鼠神经纤维瘤病1型基因启动子的转录取决于Sp1识别位点的完整性。

Transcription of the promoter of the rat NF-1 gene depends on the integrity of an Sp1 recognition site.

作者信息

Ammendola R, Gounari F, Piaggio G, De Simone V, Cortese R

机构信息

European Molecular Biology Laboratory, Heidelberg, Federal Republic of Germany.

出版信息

Mol Cell Biol. 1990 Jan;10(1):387-90. doi: 10.1128/mcb.10.1.387-390.1990.

DOI:10.1128/mcb.10.1.387-390.1990
PMID:2403642
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC360762/
Abstract

The transcription start site and promoter of the rat gene coding for the transcription factor NF-1 have been identified. The NF-1 promoter was fused to the chloramphenicol acetyltransferase-coding sequence, and the resulting plasmid was transcriptionally active in the HepG2 cell line. Footprinting and gel retardation analysis indicated that the transcription factor Sp1 binds to the NF-1 promoter. Mutants in the Sp1-binding site displayed a strong reduction in transcriptional activity.

摘要

编码转录因子NF-1的大鼠基因的转录起始位点和启动子已被确定。将NF-1启动子与氯霉素乙酰转移酶编码序列融合，所得质粒在HepG2细胞系中具有转录活性。足迹法和凝胶阻滞分析表明转录因子Sp1与NF-1启动子结合。Sp1结合位点的突变体转录活性显著降低。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a09/360762/7eadca62f761/molcellb00037-0409-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a09/360762/2ff29659e752/molcellb00037-0408-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a09/360762/ce0322cec3e9/molcellb00037-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a09/360762/7eadca62f761/molcellb00037-0409-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a09/360762/2ff29659e752/molcellb00037-0408-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a09/360762/ce0322cec3e9/molcellb00037-0409-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6a09/360762/7eadca62f761/molcellb00037-0409-b.jpg

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