Barcelona Centre for International Health Research, Hospital Clinic, Universitat de Barcelona, BarcelonaSpain, Spain.
Mem Inst Oswaldo Cruz. 2013 Sep;108(6):801-3. doi: 10.1590/0074-0276108062013019.
The production of fully functional human red cells in vitro from haematopoietic stem cells (hHSCs) has been successfully achieved. Recently, the use of hHSCs from cord blood represented a major improvement to develop the continuous culture system for Plasmodium vivax. Here, we demonstrated that CD34⁺ hHSCs from peripheral blood and bone marrow can be expanded and differentiated to reticulocytes using a novel stromal cell. Moreover, these reticulocytes and mature red blood cells express surface markers for entrance of malaria parasites contain adult haemoglobin and are also permissive to invasion by P. vivax and Plasmodium falciparum parasites.
已经成功地从造血干细胞（hHSCs）体外产生完全功能的人类红细胞。最近，使用来自脐带血的 hHSCs 代表了对开发连续培养系统用于恶性疟原虫的重大改进。在这里，我们证明了外周血和骨髓中的 CD34+ hHSCs 可以使用新型基质细胞进行扩增和分化为网织红细胞。此外，这些网织红细胞和成熟的红细胞表达表面标记物，允许恶性疟原虫和疟原虫侵入，并且含有成人血红蛋白。
