Kumar Pradeep, Singh Vinay Kumar, Singh D K
Department of Zoology, Malacology Laboratory, DDU Gorakhpur University, Gorakhpur, UP, India.
Rev Inst Med Trop Sao Paulo. 2011 Sep-Oct;53(5):271-5. doi: 10.1590/s0036-46652011000500006.
The effect of sub-lethal feeding of bait formulations containing molluscicidal component of Ferula asafoetida (ferulic acid, umbelliferone), Syzygium aromaticum (eugenol) and Carum carvi (limonene) on biochemical changes in the ovotestis of snail Lymnaea acuminata were studied. Bait formulations feeding to L. acuminata were studied in clear glass aquaria having diameter of 30 cm. Baits were prepared from different binary combinations of attractant amino acid (valine, aspartic acid, lysine and alanine 10 mM) in 100 mL of 2% agar solution + sub-lethal (20% and 60% of 24h LC50) doses of different molluscicides (ferulic acid, umbelliferone, eugenol and limonene). These baits caused maximum significant reduction in free amino acid, protein, DNA, RNA levels i.e. 41.37, 23.56, 48.36 and 14.29% of control in the ovotestis of the snail, respectively. Discontinuation of feeding after treatment of 60% of 96h LC50 of molluscicide containing bait for next 72h caused a significant recovery in free amino acid, protein, DNA and RNA levels in the ovotestis of L. acuminata.
研究了含有阿魏（阿魏酸、伞形花内酯）、丁香（丁香酚）和葛缕子（柠檬烯）杀螺成分的诱饵制剂亚致死投喂对尖膀胱螺卵巢精巢生化变化的影响。在直径为30厘米的透明玻璃水族箱中研究了对尖膀胱螺投喂诱饵制剂的情况。诱饵由100毫升2%琼脂溶液中引诱氨基酸（缬氨酸、天冬氨酸、赖氨酸和丙氨酸，10毫摩尔）的不同二元组合 + 不同杀螺剂（阿魏酸、伞形花内酯、丁香酚和柠檬烯）的亚致死剂量（24小时半数致死浓度的20%和60%）制备而成。这些诱饵导致尖膀胱螺卵巢精巢中的游离氨基酸、蛋白质、DNA、RNA水平显著降低，分别为对照的41.37%、23.56%、48.36%和14.29%。在用含60% 96小时半数致死浓度杀螺剂的诱饵处理后停止投喂72小时，尖膀胱螺卵巢精巢中的游离氨基酸、蛋白质、DNA和RNA水平显著恢复。
