Avila Luciana Farias da Costa de, Telmo Paula de Lima, Martins Lourdes Helena Rodrigues, Glaeser Thaís Aimeé, Conceição Fabricio Rochedo, Leite Fábio Pereira Leivas, Scaini Carlos James
Universidade Federal de Pelotas, 96010-900PelotasRSBrazilPost-Graduate Program in Parasitology, Universidade Federal de Pelotas (UFPEL); Campus Universitário, Caixa Postal 354, 96010-900 Pelotas, RS, Brazil. Tel.: 55.53.3275-7350, Fax: 55.53.3275-7350.
Rev Inst Med Trop Sao Paulo. 2013 Sep-Oct;55(5):363-5. doi: 10.1590/S0036-46652013000500012.
In a previous study our group found that the probiotic Saccharomyces boulardii was capable of reducing the intensity of infection in mice with toxocariasis. In order to assess whether the mechanism involved would be a direct action of the probiotic on Toxocara canis larvae, this study was designed. Both probiotics were singly cultivated in plates containing RPMI 1640 medium and T. canis larvae. S. boulardii and B. cereus var. toyoi cultures presented 97.6% and 95.7% of larvae with positive motility, respectively, and absence of color by the dye trypan blue, not representing significant difference to the control group (p > 0.05). We conclude that none of the probiotics showed in vitro effects on T. canis larvae and that the interaction with the intestinal mucosa is necessary for the development of the protective effect of S. boulardii.
在先前的一项研究中，我们团队发现益生菌布拉酵母菌能够降低患弓蛔虫病小鼠的感染强度。为了评估其中涉及的机制是否是益生菌对犬弓蛔虫幼虫的直接作用，我们设计了本研究。将两种益生菌分别在含有RPMI 1640培养基和犬弓蛔虫幼虫的培养皿中培养。布拉酵母菌和蜡样芽孢杆菌var. toyoi培养物中，分别有97.6%和95.7%的幼虫具有活跃的运动能力，且经台盼蓝染色后无颜色变化，与对照组相比无显著差异（p>0.05）。我们得出结论，两种益生菌在体外对犬弓蛔虫幼虫均无作用，且布拉酵母菌发挥保护作用需要与肠黏膜相互作用。
