入侵者：利用由2'-O-（芘-1-基）甲基核糖核苷酸的链间拉链修饰的双链体识别双链DNA。
Invaders: Recognition of Double-Stranded DNA by Using Duplexes Modified with Interstrand Zippers of 2'-O-(Pyren-1-yl)methyl-ribonucleotides.
作者信息
Didion Bradley A, Karmakar Saswata, Guenther Dale C, Sau Sujay P, Verstegen John P, Hrdlicka Patrick J
机构信息
Minitube of America, Inc. PO Box 930187, 419 Venture Ct., Verona, WI 53593 (USA).
出版信息
Chembiochem. 2013 Sep 2;14(13):1534-1538. doi: 10.1002/cbic.201300414. Epub 2013 Aug 23.
Abstract
The invasion has begun: Invaders are shown to recognize DNA hairpins in cell-free assays and chromosomal DNA during non-denaturing fluorescence in situ hybridization (nd-FISH) experiments. As Invaders are devoid of inherent sequence limitations, many previously inaccessible DNA targets could become accessible to exogenous control with important ramifications for karyotyping, in vivo imaging, and gene regulation.
摘要
入侵已经开始
在非变性荧光原位杂交（nd-FISH）实验中，入侵者在无细胞检测和染色体DNA中被证明能识别DNA发夹结构。由于入侵者没有内在的序列限制，许多以前无法接近的DNA靶点可能会受到外源控制，这对核型分析、体内成像和基因调控具有重要影响。
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