Shrestha Bhushan, Choi Sung-Keun, Kim Ho-Kyoung, Kim Tae-Woong, Sung Jae-Mo
Entomopathogenic Fungal Culture Collection (EFCC), Department of Applied Biology, Kangwon National University, Chuncheon 200-701, Korea.
Mycobiology. 2005 Sep;33(3):125-30. doi: 10.4489/MYCO.2005.33.3.125. Epub 2005 Sep 30.
Pigmentation of ascospore-derived isolates from seven different natural specimens of Cordyceps militaris EFCC C-5888, EFCC C-7159, EFCC C-7833, EFCC C-7991, EFCC C-8021, EFCC C-8023 and EFCC C-8179 was observed on the plates of Sabouraud Dextrose agar plus Yeast Extract at 25℃ under continuous illumination (500 lux). Pigmentation of the wild-type isolates of C. militaris was diverse ranging from yellowish white to orange, while white color was believed as a mutant. Inheritance of pigmentation was found to be controlled by both parental isolates when F1 progeny were analyzed. Pigmentation and mating type were shown to be either independent or distantly linked each other due to the high percentage of non-parental phenotypes among F1 progeny. Crosses between white mutant isolates of C. militaris yielded progeny with wild type pigmentations, indicating that the albino mutations in the parents were unlinked to each other.
在25℃持续光照（500勒克斯）条件下，将来自7个不同天然蛹虫草标本（EFCC C - 5888、EFCC C - 7159、EFCC C - 7833、EFCC C - 7991、EFCC C - 8021、EFCC C - 8023和EFCC C - 8179）的子囊孢子衍生分离株接种在含酵母提取物的沙氏葡萄糖琼脂平板上，观察到色素沉着现象。蛹虫草野生型分离株的色素沉着各不相同，从黄白色到橙色不等，而白色被认为是一种突变体。分析F1代子代时发现，色素沉着的遗传由双亲分离株共同控制。由于F1代子代中非亲本表型的比例较高，色素沉着和交配型显示为相互独立或远距离连锁。蛹虫草白色突变体分离株之间的杂交产生了具有野生型色素沉着的子代，这表明亲本中的白化突变彼此不连锁。
