Geis P A, Wheeler M H, Szaniszlo P J
Arch Microbiol. 1984 Apr;137(4):324-8. doi: 10.1007/BF00410729.
Melanin synthesis in the dematiaceous, polymorphic hyphomycete Wangiella dermatitidis, a human pathogen, was investigated by biochemical and physiological techniques. Mutants with a decrease or loss in melanin synthesis were induced and isolated. Melanin precursors were obtained from the mutants, purified, and then identified by comparison with authentic compounds from Verticillium dahliae . Isolation of scytalone , vermelone , flaviolin , and 1,8- dihydroxynaphthalene from the mutants of Wangiella dermatitidis, and cross-feeding of the mutants with those of Verticillium dahliae indicated that melanin synthesis in this organism took place by the pentaketide pathway. Melanin that formed in cell walls of an albino mutant treated with scytalone was identified in appearance to that in cell walls of the wild-type strain. This also suggested that pentaketide synthesis of melanin occurred in the fungus.
利用生化和生理学技术，对人类病原菌、多形性丝状真菌皮炎万吉拉菌（Wangiella dermatitidis）中的黑色素合成进行了研究。诱导并分离出黑色素合成减少或缺失的突变体。从突变体中获得黑色素前体，进行纯化，然后通过与来自大丽轮枝菌（Verticillium dahliae）的标准化合物进行比较来鉴定。从皮炎万吉拉菌突变体中分离出了鬼臼毒素、紫罗酮、黄萎菌素和1,8 - 二羟基萘，并且用大丽轮枝菌的突变体对皮炎万吉拉菌的突变体进行交叉饲养，结果表明该生物体中的黑色素合成通过戊酮糖途径进行。用鬼臼毒素处理的白化突变体细胞壁中形成的黑色素，在外观上与野生型菌株细胞壁中的黑色素一致。这也表明黑色素的戊酮糖合成发生在该真菌中。
