Salehi Mohammad, Kato Yoko, Tsunoda Yukio
Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, 631-8505, Japan.
Zygote. 2014 May;22(2):213-7. doi: 10.1017/S0967199413000336. Epub 2013 Sep 16.
The beneficial effect of supplementing culture medium with melatonin has been reported during in vitro embryo development of species such as mouse, bovine and porcine. However, the effect of melatonin on mouse somatic cell nuclear transfer remains unknown. In this study, we assessed the effects of various concentrations of melatonin (10-6 to 10-12 M) on the in vitro development of mouse somatic cell nuclear transfer embryos for 96 h. Embryos cultured without melatonin were used as control. There was no significant difference in cleavage rates between the groups supplemented with melatonin, dimethyl sulphoxide (DMSO) and the control. The rate of development to blastocyst stage was significantly higher in the group supplemented with 10-12 M melatonin compared with the control group (P < 0.05). Thus, our data demonstrated that adding melatonin to pre-implantation mouse nuclear-transferred embryos can accelerate blastocyst formation.
据报道，在小鼠、牛和猪等物种的体外胚胎发育过程中，向培养基中添加褪黑素具有有益效果。然而，褪黑素对小鼠体细胞核移植的影响尚不清楚。在本研究中，我们评估了不同浓度（10-6至10-12 M）的褪黑素对小鼠体细胞核移植胚胎体外发育96小时的影响。未添加褪黑素培养的胚胎用作对照。添加褪黑素、二甲基亚砜（DMSO）的组与对照组之间的分裂率没有显著差异。与对照组相比，添加10-12 M褪黑素的组发育到囊胚阶段的比率显著更高（P < 0.05）。因此，我们的数据表明，向植入前的小鼠核移植胚胎中添加褪黑素可以加速囊胚形成。
