Nagaraj Karuppiah, Arunachalam Sankaralingam
School of Chemistry, Bharathidasan University, Trichirappalli 620024, Tamilnadu, India.
Int J Biol Macromol. 2013 Nov;62:273-80. doi: 10.1016/j.ijbiomac.2013.09.002. Epub 2013 Sep 13.
The interaction of cis-Co(phen)2(HA)23, a cationic surfactant complex (phen=1,10-phenanthroline, HA=hexadecylamine), with calf thymus DNA has been studied by UV-vis absorption, fluorescence spectroscopy, cyclic voltammetry, circular dichroism, and viscosity measurements. The spectroscopic studies together with cyclic voltammetry and viscosity experiments support that the surfactant-cobalt(III) complex binds to calf thymus DNA (CT DNA) by intercalation through the aliphatic chain present in the complex into the base pairs of DNA. The presence of phenanthroline ligand with larger π-frame work may also enhance intercalation. Besides the effect of binding of surfactant cobalt(III) complex to DNA in presence of β-cyclodextrin has also studied. In presence of β-cyclodextrin the binding occurs through surface and (or) groove binding. The complex was investigated as one of the potential selective anticancer prodrugs. The complex was tested also in vitro on human monolayer tumor cell lines: HepG2 (human hepatocellular liver carcinoma).
通过紫外可见吸收光谱、荧光光谱、循环伏安法、圆二色光谱和粘度测量等方法，研究了阳离子表面活性剂配合物顺式-Co(phen)2(HA)23（phen = 1,10-菲咯啉，HA = 十六胺）与小牛胸腺DNA的相互作用。光谱研究以及循环伏安法和粘度实验表明，表面活性剂-钴(III)配合物通过配合物中存在的脂肪链插入DNA的碱基对，从而与小牛胸腺DNA（CT DNA）结合。具有较大π骨架的菲咯啉配体的存在也可能增强插入作用。此外，还研究了在β-环糊精存在下表面活性剂钴(III)配合物与DNA结合的影响。在β-环糊精存在下，结合通过表面和（或）沟槽结合发生。该配合物被作为潜在的选择性抗癌前药之一进行了研究。该配合物还在人单层肿瘤细胞系HepG2（人肝细胞肝癌）上进行了体外测试。
