Zhao Mei-Lin, Qiu Xing-Xing, Zhang Wen-Jiao, Han Dong, Cui Heng-Lin, Li Zheng-Rong
School of Food and Biological Engineering, Jiangsu University, 301 Xuefu Road, Jingkou District, Zhenjiang, 212013, People's Republic of China.
Curr Microbiol. 2014 Feb;68(2):156-60. doi: 10.1007/s00284-013-0457-8. Epub 2013 Sep 19.
Halophilic archaeal strain GX31(T) was isolated from a marine solar saltern of China. The cells of the strain were rod-shaped and lysed in distilled water, stain Gram-negative and formed red-pigmented colonies. It was neutrophilic, and required at least 0.9 M NaCl and 0-1.0 M MgCl2 for growth under the optimum growth temperature of 37 °C. The major polar lipids of the strain were phosphatidylglycerol (PG), PG phosphate methyl ester, PG sulphate, and two major glycolipids chromatographically identical to sulphated mannosyl glucosyl diether (S-DGD-1) and mannosyl glucosyl diether (DGD-1), respectively. Trace amounts of two unidentified lipids were also detected. On the basis of 16S rRNA gene sequence analysis, strain GX31(T) was closely related to the members of Halobellus of the family Halobacteriaceae with similarities of 94.1-98.7 %. Strain GX31(T) showed 89.8-95.4 % of the rpoB' gene similarity to the members of Halobellus. The DNA G+C content of strain GX31(T) was 66.8 mol%. Strain GX31(T) showed low DNA-DNA relatedness with two most related members of the genus Halobellus. The phenotypic, chemotaxonomic and phylogenetic properties suggest that strain GX31(T) represent a novel species of the genus Halobellus, for which the name Halobellus litoreus sp. nov. is proposed. The type strain is GX31(T) (=CGMCC 1.10387(T) = JCM 17118(T)).
嗜盐古菌菌株GX31(T) 是从中国的一个海洋太阳能盐场分离得到的。该菌株的细胞呈杆状，在蒸馏水中会裂解，革兰氏染色阴性，形成红色色素菌落。它是嗜中性的，在37°C的最适生长温度下生长需要至少0.9 M NaCl和0 - 1.0 M MgCl2。该菌株的主要极性脂类分别为磷脂酰甘油（PG）、PG磷酸甲酯、PG硫酸盐，以及两种在色谱上分别与硫酸化甘露糖基葡糖基二醚（S-DGD-1）和甘露糖基葡糖基二醚（DGD-1）相同的主要糖脂。还检测到微量的两种未鉴定脂类。基于16S rRNA基因序列分析，菌株GX31(T) 与盐杆菌科盐贝属的成员密切相关，相似度为94.1 - 98.7%。菌株GX31(T) 与盐贝属成员的rpoB' 基因相似度为89.8 - 95.4%。菌株GX31(T) 的DNA G+C含量为66.8 mol%。菌株GX31(T) 与盐贝属两个最相关成员的DNA-DNA相关性较低。表型、化学分类和系统发育特性表明，菌株GX31(T) 代表盐贝属的一个新物种，为此提出新名称盐滩盐贝（Halobellus litoreus sp. nov.）。模式菌株为GX31(T)（=CGMCC 1.10387(T) = JCM 17118(T)）。
