Department of Bioengineering and Nano-Bioengineering, Incheon National University, Incheon, 22012, Republic of Korea.
Biotechnology Institute, University of Minnesota, St. Paul, MN, 55108, USA.
Extremophiles. 2024 Nov 6;28(3):49. doi: 10.1007/s00792-024-01367-x.
A novel extremely halophilic archaeon designated, MBLA0158, was isolated from a solar saltern in Sorae, Republic of Korea. The colonies are red-pigmented, Gram-stain-negative, pleomorphic, non-motile, and lysed in distilled water. The strain grows at 25-45 °C (optimum, 37 °C), in 15-30% (w/v) NaCl (optimum, 20%) and 0.1-1.0 M Mg (optimum, 0.2-0.3 M) at pH 6.0-10.0 (optimum, 7.0-8.0). Comparative analysis based on the 16S rRNA gene sequence revealed that this strain is most closely related to the Halobellus inordinatus YC20 with a sequence identity of 96.0%. Strain MBLA0158 contained phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester as major polar lipids. The genome size is 3.29 Mb and the DNA G + C content is 66.9 mol%. Phylogenomic analysis confirmed that strain MBLA0158 is distinct from previously reported type strains of the genus Halobellus. Pan-genome analysis showed that strain MBLA0158 contains 419 genes that are not present in other type strains of the genus Halobellus. Based on overall analyses, strain MBLA0158 is considered to represent a new species of the genus Halobellus, for which the name Halobellus rubicundus sp. nov. is proposed. The type strain is MBLA0158 (= KCTC 4318 = JCM 36642).
一株新型极端嗜盐古菌，命名为 MBLA0158，从韩国 Sorae 的一个太阳能盐场中分离得到。菌落呈红色，革兰氏阴性，形态多形，不运动，在蒸馏水中溶解。该菌株在 25-45°C（最佳温度为 37°C）、15-30%（w/v）NaCl（最佳浓度为 20%）和 0.1-1.0 M Mg（最佳浓度为 0.2-0.3 M）、pH6.0-10.0（最佳 pH 值为 7.0-8.0）的条件下生长。基于 16S rRNA 基因序列的比较分析表明，该菌株与 Halobellus inordinatus YC20 最为密切相关，序列相似度为 96.0%。菌株 MBLA0158 含有磷脂酰甘油和磷脂酰甘油磷酸甲基酯作为主要极性脂。基因组大小为 3.29 Mb，DNA G+C 含量为 66.9 mol%。系统发育基因组分析证实，菌株 MBLA0158 与先前报道的 Halobellus 属的模式菌株明显不同。泛基因组分析表明，菌株 MBLA0158 含有 419 个不在其他 Halobellus 属模式菌株中存在的基因。综合分析表明，菌株 MBLA0158 代表了 Halobellus 属的一个新种，建议命名为 Halobellus rubicundus sp. nov.。其模式菌株为 MBLA0158（=KCTC 4318=JCM 36642）。
