Cha In-Tae, Yim Kyung June, Song Hye Seon, Lee Hae-Won, Hyun Dong-Wook, Kim Kil-Nam, Seo Myung-Ji, Kim Daekyung, Choi Jong-Soon, Lee Sung-Jae, Bae Jin-Woo, Rhee Sung-Keun, Choi Hak-Jong, Rhee Jin-Kyu, Nam Young-Do, Roh Seong Woon
Korea Basic Science Institute, Daejeon, 305-806, Republic of Korea.
Antonie Van Leeuwenhoek. 2014 May;105(5):925-32. doi: 10.1007/s10482-014-0147-y. Epub 2014 Mar 9.
A halophilic archaeon, designed strain CBA1103(T), was isolated from non-purified solar salt. The cells of strain CBA1103(T) were observed to be Gram-stain negative and pleomorphic, and the colonies appear red. Strain CBA1103(T) was observed to grow between 20 and 55 °C (optimum 37 °C), and in NaCl concentrations of 10-30 % (w/v) (optimum 15 %) with 0-0.5 M MgSO4·7H2O (optimum 0.1 M) and at pH 6.0-9.0 (optimum pH 7.0). Additionally, the cells lyse in distilled water. The major polar lipids of strain CBA1103(T) are phosphatidylglycerol, phosphatidylglycerol phosphate methyl ester, phosphatidylglycerol sulfate and two glycolipids chromatographically identical to sulfated mannosyl glucosyl diether and manosyl glucosyl diether. Strain CBA1103(T) is shown to belong to the Halobellus genus and exhibits similarity to related taxa; the 16S rRNA gene sequence similarity between strain CBA1103(T) and Halobellus rarus 18362(T), Hbs. limi 16811(T), Hbs. litoreus JCM 17118(T), Hbs. inordinatus YC20(T), Hbs. clavatus TNN18(T) and Hbs. salinus CSW2.24.4(T) is 97.3, 96.5, 96.5, 94.5, 94.5 and 93.7 %, respectively. The RNA polymerase subunit B gene sequence of strain CBA1103(T) shows 93.7 % similarity with the sequence of Hbs. litoreus JCM 17118(T); the similarity is lower with sequences from the type strains of other species of Halobellus. The genomic DNA G+C content of strain CBA1103(T) was determined to be 67.0 mol% a value which is in the range of the genomic DNA G+C content of members of the genus Halobellus (61.5-69.2 mol%). These results suggest that strain CBA1103(T) should be considered to represent a new taxon for which the name Halobellus rufus sp. nov. is proposed, with the type strain CBA1103(T) (=CECT 8423(T) =JCM 19434(T)).
从未提纯的日晒盐中分离出一株嗜盐古菌，命名为菌株CBA1103(T)。观察到菌株CBA1103(T)的细胞革兰氏染色阴性且形态多样，菌落呈红色。观察到菌株CBA1103(T)在20至55°C（最适温度37°C）之间生长，NaCl浓度为10 - 30%（w/v）（最适浓度15%），MgSO4·7H2O浓度为0 - 0.5 M（最适浓度0.1 M），pH值为6.0 - 9.0（最适pH值7.0）时生长。此外，该细胞在蒸馏水中会裂解。菌株CBA1103(T)的主要极性脂类为磷脂酰甘油、磷脂酰甘油磷酸甲酯、磷脂酰甘油硫酸酯以及两种与硫酸化甘露糖基葡糖基二醚和甘露糖基葡糖基二醚色谱相同的糖脂。菌株CBA1103(T)被证明属于盐铃菌属，与相关分类单元具有相似性；菌株CBA1103(T)与稀有盐铃菌18362(T)、盐铃菌16811(T)、滨海盐铃菌JCM 17118(T)、异常盐铃菌YC20(T)、棒状盐铃菌TNN18(T)和盐生盐铃菌CSW2.24.4(T)的16S rRNA基因序列相似性分别为97.3%、96.5%、96.5%、94.5%、94.5%和93.7%。菌株CBA1103(T)的RNA聚合酶亚基B基因序列与滨海盐铃菌JCM 17118(T)的序列相似性为93.7%；与盐铃菌属其他种模式菌株的序列相似性较低。菌株CBA1103(T)的基因组DNA G + C含量测定为67.0 mol%，该值在盐铃菌属成员基因组DNA G + C含量范围（61.5 - 69.2 mol%）内。这些结果表明，菌株CBA1103(T)应被视为代表一个新的分类单元，为此提出新名称盐红盐铃菌（Halobellus rufus sp. nov.），模式菌株为CBA1103(T)（=CECT 8423(T) =JCM 19434(T)）。
