Institute of Life Sciences, Department of Biotechnology, Nalco Square, Bhubaneswar-751 023, India.
Int J Syst Evol Microbiol. 2014 Jan;64(Pt 1):228-235. doi: 10.1099/ijs.0.056424-0. Epub 2013 Sep 18.
Comparative phenotypic, chemotaxonomic and genetic analysis revealed significant similarities among strains of the genera Tepidiphilus and Petrobacter. Analysis of 16S rRNA gene sequences and DNA-DNA relatedness of the type strains Tepidiphilus margaritifer N2-214(T) and Petrobacter succinatimandens 4BON(T) showed sequence similarity of 98.9 % and less than 40 % relatedness, indicating that these strains represent different species of same genus. Both strains had phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and diphosphatidylglycerol as major polar lipids. Their fatty acid profiles were almost identical, with the predominant fatty acids C16 : 0, C17 : 0 cyclo and C19 : 0 cyclo ω8c. In view of this, we propose to transfer the member of the genus Petrobacter to the genus Tepidiphilus as Tepidiphilus succinatimandens comb. nov. and to emend the description of the genus Tepidiphilus. Further, a novel bacterium, strain JHK30(T), was isolated from a terrestrial hot spring located at Jharkhand, India, and was identified following a polyphasic approach. Cells were non-sporulating, aerobic, Gram-stain-negative rods and motile by a single polar flagellum. Optimum temperature for growth was 50-55 °C at pH 6.5-7.0. 16S rRNA gene sequence analysis revealed 99.71 % similarity with P. succinatimandens 4BON(T) ( = DSM 15512(T)) and 98.71 % with T. margaritifer N2-214(T) ( = DSM 15129(T)). However, DNA-DNA relatedness of strain JHK30(T) with these two type strains was well below 70 %. The DNA G+C base composition was 66.1 mol%. Strain JHK30(T) represents a novel species of the genus Tepidiphilus for which the name Tepidiphilus thermophilus sp. nov. is proposed. The type strain is JHK30(T) ( = JCM 19170(T) = LMG 27587(T)= DSM 27220(T)).
比较表型、化学生态和遗传分析表明，Tepidiphilus 和 Petrobacter 属的菌株之间存在显著的相似性。对模式菌株 Tepidiphilus margaritifer N2-214(T) 和 Petrobacter succinatimandens 4BON(T) 的 16S rRNA 基因序列分析和 DNA-DNA 相关性表明，它们的序列相似性为 98.9%，相关性小于 40%，表明这些菌株代表同一属的不同种。这两个菌株都有磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱和双磷脂酰甘油作为主要的极性脂。它们的脂肪酸图谱几乎相同，主要脂肪酸为 C16:0、C17:0 环和 C19:0 环 ω8c。鉴于此，我们建议将 Petrobacter 属的成员转移到 Tepidiphilus 属中，作为 Tepidiphilus succinatimandens comb. nov.，并对 Tepidiphilus 属的描述进行修正。此外，从印度恰尔康得邦的一个陆地温泉中分离到一株新的细菌，菌株 JHK30(T)，并通过多相方法进行鉴定。细胞是非孢子形成的、需氧的、革兰氏阴性棒状菌，通过单一极鞭毛运动。最适生长温度为 50-55°C，pH 值为 6.5-7.0。16S rRNA 基因序列分析表明，它与 P. succinatimandens 4BON(T)(=DSM 15512(T))的相似度为 99.71%，与 T. margaritifer N2-214(T)(=DSM 15129(T))的相似度为 98.71%。然而，菌株 JHK30(T)与这两个模式菌株的 DNA-DNA 相关性低于 70%。DNA G+C 碱基组成为 66.1 mol%。菌株 JHK30(T)代表 Tepidiphilus 属的一个新种，建议将其命名为 Tepidiphilus thermophilus sp. nov.。模式菌株为 JHK30(T)(=JCM 19170(T)=LMG 27587(T)=DSM 27220(T))。
