Kim Gi-Young, Kim Seung-Hoon, Hwang Sue-Yun, Kim Ho-Youn, Park Yeong-Min, Park Soon-Kew, Lee Min-Ki, Lee Sang-Hwa, Lee Tae-Ho, Lee Jae-Dong
Department of Microbiology, College of Natural Sciences, Pusan National University, Pusan 609-735, South Korea.
Biol Pharm Bull. 2003 Jun;26(6):823-31. doi: 10.1248/bpb.26.823.
To examine whether oral administration of proteoglycan derived from Phellinus linteus, which is known as the medicinal mushroom, can prevent or treat collagen-induced arthritis (CIA) in mice as experimental model of autoimmune disease. CIA was induced by intradermal injection of type II collagen (CII) emulsified with complete freund's adjuvant (CFA) into the base of the tail (on day 7) followed by a booster injection on day 21 into the footpad. To examine the ability of proteoglycan to effect the inhibition of CIA, doses of proteoglycan were orally administered on day 0 (pre-administration) or day 28 (post-administration) at two groups. The inhibition of CIA by oral administration of proteoglycan was associated with decrease in anti-CII IgG and IgG2a antibodies (Abs) as well as varying kinds of cytokines including IL-12, TNF-alpha, and IFN-gamma. The results showed that administration of proteoglycan was followed by decrease of CIA of the mice in pre- and post-administration groups. Our findings suggest that immunomodulating proteoglycan isolated from P. linteus may be crucially involved in the prevention and treatment of autoimmune joint inflammation such as rheumatoid arthritis, although no definite role of anti-CII Abs in the human disease has been established.
为了研究口服来源于桑黄（一种著名的药用蘑菇）的蛋白聚糖是否能够预防或治疗作为自身免疫性疾病实验模型的小鼠胶原诱导性关节炎（CIA）。通过在小鼠尾巴根部皮内注射用完全弗氏佐剂（CFA）乳化的II型胶原（CII）（第7天），并在第21天在足垫进行加强注射来诱导CIA。为了研究蛋白聚糖抑制CIA的能力，在两组中分别于第0天（给药前）或第28天（给药后）口服不同剂量的蛋白聚糖。口服蛋白聚糖对CIA的抑制作用与抗CII IgG和IgG2a抗体（Abs）以及包括IL-12、TNF-α和IFN-γ在内的多种细胞因子的减少有关。结果表明，给药前和给药后组的小鼠在口服蛋白聚糖后CIA均有所减轻。我们的研究结果表明，从桑黄中分离出的具有免疫调节作用的蛋白聚糖可能在预防和治疗类风湿关节炎等自身免疫性关节炎症中起关键作用，尽管抗CII Abs在人类疾病中的明确作用尚未确定。
