Turaga Diwakar, Holy Timothy E
Dept. of Anatomy & Neurobiology, Washington University in St. Louis School of Medicine, St. Louis, MO 63110, USA.
Biomed Opt Express. 2013 Aug 14;4(9):1654-61. doi: 10.1364/BOE.4.001654. eCollection 2013.
Light sheet microscopy allows rapid imaging of three-dimensional fluorescent samples, using illumination and detection axes that are orthogonal. For imaging large samples, this often forces the objective to be tilted relative to the sample's surface; for samples that are not precisely matched to the immersion medium index, this tilt introduces aberrations. Here we calculate the nature of these aberrations for a simple tissue model, and show that a low-dimensional parametrization of these aberrations facilitates online correction via a deformable mirror without introduction of beads or other fiducial markers. We use this approach to demonstrate improved image quality in living tissue.
光片显微镜术能够对三维荧光样本进行快速成像，其照明轴和检测轴相互正交。对于大样本成像，这通常会迫使物镜相对于样本表面倾斜；对于折射率与浸没介质不完全匹配的样本，这种倾斜会引入像差。在此，我们针对一个简单的组织模型计算了这些像差的性质，并表明对这些像差进行低维参数化有助于通过可变形镜进行在线校正，而无需引入珠子或其他基准标记。我们使用这种方法证明了在活体组织中图像质量得到了改善。
