Departamento de Estudos Básicos e Instrumentais, Universidade Estadual do Sudoeste da Bahia, 45700-000 Itapetinga, BA, Brazil.
J Ethnopharmacol. 2013 Nov 25;150(2):595-600. doi: 10.1016/j.jep.2013.09.005. Epub 2013 Sep 16.
In traditional medicine, teas made from leaves and bark of Gallesia gorazema are used as antispasmodic, anthelmintic, antihemorrhagic and febrifuge agents. Crude leaves of this plant are also employed as a remedy in the treatment of abscesses, orchitis, gonorrhea and for rheumatic pain relief. this study investigates the presumed antinociceptive and anti-inflammatory activities of leaves and roots Gallesia gorazema (Phytolaccaceae) extracts. The most active extract and its isolated compound, a new natural product, are also evaluated against viruses HSV-1 and HSV-2.
In vivo experiments with mice were used to assess the analgesic and anti-inflammatory activities of Gallesia gorazema extracts. Antiviral activity of extracts and the new natural product was investigated by in vitro experiments.
Results show that dichloromethanic root (DRE) and ethanolic leaf (ELE) extracts displayed significant antinociceptive and anti-inflammatory activities in in vivo experiments with mice. Both extracts were also assayed against the herpes simplex viruses HSV-1 and HSV-2, but only DRE was highly active, showing a selective antiviral effect against HSV-1. Phytochemical fractionation of DRE led to the isolation of 28-hydroxyoctacosyl ferulate, a novel natural product, which displayed strong antiviral activity against HSV-1 (EC₅₀=21.6 μg/mL) with a selective index above 9, justifying, at least in part, the high selective antiviral activity observed for DRE.
These results suggest that the plant Gallesia gorazema is a potential candidate for the development of novel anti-herpetic phytomedicines.
在传统医学中，由 Gallesia gorazema 的叶子和树皮制成的茶被用作抗痉挛、驱虫、止血和解热剂。这种植物的粗叶也被用作治疗脓肿、睾丸炎、淋病和缓解风湿疼痛的药物。本研究调查了 Gallesia gorazema（商陆科）叶和根提取物的假定镇痛和抗炎活性。最活跃的提取物及其分离的化合物，一种新的天然产物，也针对 HSV-1 和 HSV-2 病毒进行了评估。
使用小鼠体内实验来评估 Gallesia gorazema 提取物的镇痛和抗炎活性。通过体外实验研究了提取物和新天然产物的抗病毒活性。
结果表明，二氯甲烷根（DRE）和乙醇叶（ELE）提取物在小鼠体内实验中表现出显著的镇痛和抗炎活性。这两种提取物也针对单纯疱疹病毒 HSV-1 和 HSV-2 进行了测试，但只有 DRE 具有高度活性，对 HSV-1 表现出选择性抗病毒作用。DRE 的植物化学分级分离导致分离出 28-羟基二十八烷基咖啡酸酯，这是一种新型天然产物，对 HSV-1 具有很强的抗病毒活性（EC₅₀=21.6 μg/mL），选择性指数超过 9，这至少部分解释了 DRE 观察到的高选择性抗病毒活性。
这些结果表明，植物 Gallesia gorazema 是开发新型抗疱疹植物药的潜在候选者。
