Universidad Autónoma del Estado de Morelos Universidad Autónoma del Estado de Morelos (UAEM) Calle Iztaccihuatl Esq. Leñeros, Col. Volcanes, Cuernavaca, Morelos CP. 62350, Mexico.
J Ethnopharmacol. 2012 Dec 18;144(3):712-9. doi: 10.1016/j.jep.2012.10.018. Epub 2012 Oct 24.
Bougainvillea xbuttiana is widely distributed in Mexico and it is used as an analgesic in folk medicine.
In the present study the in vivo antinociceptive and anti-inflammatory effects of the Bougainvillea xbuttiana ethanolic extract have been studied in mice.
The phytochemical analysis was performed. Antinociceptive activity was evaluated through writhing and formalin test in mice. The anti-inflammatory activity was determined with the carrageenan-induced mice paw oedema model. IL-6, IL-10 and IFN-γ levels were determined by enzyme-like immunosorbent assay, whereas TNF and nitrite levels were detected by standard assay with L929 cells and colorimetric Griess reactive, respectively.
The results showed that the ethanolic extract of the Bougainvillea xbuttiana has significant anti-inflammatory and antinociceptive activities, by inhibition of nociception induced by acetic acid and paw oedema. This extract also induced a decrease in TNF levels and an increase of IL-6, IFN-γ and NO levels that we observed up to 2h. The highest levels of IL-10 were observed up to 4h. The ratios of pro-/anti-inflammatory cytokines in sera from mice injected with the ethanolic extract, may be manifesting an anti-inflammatory status.
The present study provides convincing evidences that Bougainvillea xbuttiana extract possesses significant anti-nociceptive and anti-inflammatory effects.
叶子花 xbuttiana 广泛分布于墨西哥，在民间医学中被用作镇痛药。
本研究旨在研究叶子花 xbuttiana 乙醇提取物在体内的镇痛和抗炎作用。
进行了植物化学分析。通过扭体和福尔马林试验评估了镇痛活性。通过角叉菜胶诱导的小鼠足肿胀模型测定抗炎活性。通过酶联免疫吸附试验测定白细胞介素 6（IL-6）、白细胞介素 10（IL-10）和干扰素 γ（IFN-γ）水平，通过 L929 细胞的标准测定和比色格里希反应分别检测肿瘤坏死因子（TNF）和亚硝酸盐水平。
结果表明，叶子花 xbuttiana 的乙醇提取物具有显著的抗炎和镇痛活性，可抑制乙酸引起的疼痛和足肿胀。该提取物还可降低 TNF 水平，增加 IL-6、IFN-γ 和 NO 水平，我们观察到这一作用可持续至 2 小时。IL-10 的最高水平可持续至 4 小时。用乙醇提取物处理的小鼠血清中的促炎/抗炎细胞因子比值可能表现出抗炎状态。
本研究提供了令人信服的证据，表明叶子花 xbuttiana 提取物具有显著的镇痛和抗炎作用。
