Spreng 危害的花、叶和果实的细胞抗氧化、抗炎和抗增殖活性

Cellular Antioxidant, Anti-Inflammatory, and Antiproliferative Activities from the Flowers, Leaves and Fruits of Spreng Harms.

机构信息

Graduate Program in Biotechnology Applied to Agriculture, Universidade Paranaense, Umuarama 87502-210, Brazil.

Graduate Program in Animal Science with Emphasis on Bioactive Products, Universidade Paranaense, Umuarama 87502-210, Brazil.

出版信息

Molecules. 2023 Jul 14;28(14):5406. doi: 10.3390/molecules28145406.

DOI:10.3390/molecules28145406

PMID:37513277

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC10383254/

Abstract

a notable species in the Atlantic Forest, has been traditionally employed in folk medicine for treating rheumatism, asthma, and worms. This study investigated the cellular antioxidant, antiproliferative, and anti-inflammatory activities of the essential oils (EOs) and crude extracts (CEs) from flowers, fruits, and leaves. The chemical identification of EOs was performed by GC-MS and CEs by UHPLC-MS. Cellular antioxidant and anti-inflammatory activities were assessed through mouse macrophage cell culture. In addition, the antiproliferative potential was evaluated in gastric, colorectal, breast, and lung tumor cell lines and non-tumor VERO cells. EOs predominantly contained organosulfur compounds in flowers (96.29%), fruits (94.94%), and leaves (90.72%). We found the main compound is 2,2'-Disulfanediyldiethanethiol in the EOs of flowers (47.00%), leaves (41.82%), and fruits (44.39%). Phenolic compounds were identified in CEs. The EOs and CEs demonstrated potential against the tumor cell lines tested (GI between 51 and 230 µg/mL). The selectivity index values were greater than 1.0 (1.01 to 3.37), suggesting a relative safety profile. Moreover, the anti-inflammatory activity IC ranged from 36.00 to 268 µg/mL, and the cellular oxidation inhibition ranged from 69% to 82%. The results suggest that oils and extracts derived from have potential for use in various industrial sectors.

摘要

一种在大西洋森林中显著的物种，传统上被用于民间医学治疗风湿、哮喘和蠕虫。本研究调查了花、果和叶的精油（EOs）和粗提取物（CEs）的细胞抗氧化、抗增殖和抗炎活性。EOs 通过 GC-MS 进行化学鉴定，CEs 通过 UHPLC-MS 进行鉴定。通过小鼠巨噬细胞培养评估细胞抗氧化和抗炎活性。此外，在胃、结直肠、乳腺和肺癌肿瘤细胞系以及非肿瘤 VERO 细胞中评估了抗增殖潜力。EOs 在花（96.29%）、果（94.94%）和叶（90.72%）中主要含有含硫化合物。我们发现 EOs 中的主要化合物是花（47.00%）、叶（41.82%）和果（44.39%）中的 2,2'-Disulfanediyldiethanethiol。CEs 中鉴定出了酚类化合物。EOs 和 CEs 对测试的肿瘤细胞系表现出潜在的作用（GI 为 51 至 230 µg/mL）。选择性指数值大于 1.0（1.01 至 3.37），表明相对安全。此外，抗炎活性 IC 范围为 36.00 至 268 µg/mL，细胞氧化抑制率范围为 69%至 82%。结果表明，从中提取的油和提取物具有在各个工业领域应用的潜力。