College of Agronomy, Shanxi Agricultural University, Taigu, China ; Key Laboratory of Ministry of Agriculture on Agro-environment and Climate Change, Institute of Environment and Sustainable Development in Agriculture (IEDA), Chinese Academy of Agricultural Sciences, Beijing, China ; Key Laboratory of Crop Gene Resources and Germplasm Enhancement on Loess Plateau, Ministry of Agriculture, Institute of Crop Genetic Resources, Shanxi Academy of Agricultural Sciences, Taiyuan, China.
PLoS One. 2013 Sep 18;8(9):e74600. doi: 10.1371/journal.pone.0074600. eCollection 2013.
Traditional Chinese medicine relies heavily on herbs, yet there is no information on how these herb plants would respond to climate change. In order to gain insight into such response, we studied the effect of elevated [CO2] on Isatis indigotica Fort, one of the most popular Chinese herb plants. The changes in leaf photosynthesis, chlorophyll fluorescence, leaf ultrastructure and biomass yield in response to elevated [CO2] (550±19 µmol mol(-1)) were determined at the Free-Air Carbon dioxide Enrichment (FACE) experimental facility in North China. Photosynthetic ability of I. indigotica was improved under elevated [CO2]. Elevated [CO2] increased net photosynthetic rate (P N), water use efficiency (WUE) and maximum rate of electron transport (J max) of upper most fully-expended leaves, but not stomatal conductance (gs), transpiration ratio (Tr) and maximum velocity of carboxylation (V c,max). Elevated [CO2] significantly increased leaf intrinsic efficiency of PSII (Fv'/Fm') and quantum yield of PSII(ΦPS II ), but decreased leaf non-photochemical quenching (NPQ), and did not affect leaf proportion of open PSII reaction centers (qP) and maximum quantum efficiency of PSII (Fv/Fm). The structural chloroplast membrane, grana layer and stroma thylakoid membranes were intact under elevated [CO2], though more starch grains were accumulated within the chloroplasts than that of under ambient [CO2]. While the yield of I. indigotica was higher due to the improved photosynthesis under elevated [CO2], the content of adenosine, one of the functional ingredients in indigowoad root was not affected.
中药主要依赖草药，但对于这些草药植物如何应对气候变化，目前还没有信息。为了深入了解这种反应，我们研究了 [CO2] 升高对菘蓝（一种最受欢迎的中药植物之一）的影响。在华北的自由空气二氧化碳增浓（FACE）实验设施中，测定了 [CO2]（550±19 µmol mol(-1)) 升高对叶片光合作用、叶绿素荧光、叶片超微结构和生物量产量的影响。菘蓝的光合能力在 [CO2] 升高下得到提高。[CO2] 升高增加了最上面完全展开叶片的净光合速率（P N）、水分利用效率（WUE）和最大电子传递速率（J max），但不影响气孔导度（gs）、蒸腾比率（Tr）和羧化最大速率（V c,max）。[CO2] 升高显著增加了叶片 PSII 的内在效率（Fv'/Fm'）和 PSII 的量子产量（ΦPS II ），但降低了叶片非光化学猝灭（NPQ），并对叶片开放 PSII 反应中心比例（qP）和 PSII 的最大量子效率（Fv/Fm）没有影响。在 [CO2] 升高下，叶绿体膜、基粒层和基质类囊体膜的结构完整，尽管叶绿体中积累的淀粉粒比大气 [CO2] 下的更多。虽然由于 [CO2] 升高下光合作用的提高，菘蓝的产量更高，但其中一种功能成分腺苷的含量不受影响。
