Zhou Ye, Li Pei-Fu, Zhang Qing-Wei, Zhang Gong, He Li-Lin, Zhang Bo-Li
Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2013 Jul;33(7):1791-5.
The common peak ratio and variant peak ratio were calculated by FTIR spectroscopy of six medicinal plants of polygonatum. The index sequence of common peak ratio and variant peak ratio of six medicinal plants was established and the FTIR spectroscopy was analyzed by cluster analysis, which indicated the sibship of the six medicinal plants, polygonati rhizoma, polygonati odorati rhizoma and their adulterant such as Polygonatum filipes Merr., Polygonatum macropodium Turcz. and Polygonatum humile Fisch Ex Maxim. could be identified by comparing second derivative spectrum of the samples. FTIR with good characteristics such as simple and rapid procedures, nondestructive testing, small amount of sample required, good reproducibility and specificity of fingerprint, provides a new method for identification of polygonati rhizoma, polygonati odorati rhizoma and their adulterant.
采用傅里叶变换红外光谱法(FTIR)测定了6种黄精属药用植物的共有峰率和变异峰率。建立了6种药用植物共有峰率和变异峰率的指标序列,并采用聚类分析法对FTIR光谱进行分析,结果表明通过比较样品的二阶导数光谱可鉴别黄精、玉竹及其混淆品如多花黄精、长梗黄精和小玉竹之间的亲缘关系。FTIR具有操作简单快速、无损检测、样品用量少、重现性好、指纹图谱特异性强等优点,为黄精、玉竹及其混淆品的鉴别提供了一种新方法。