解析相关基因组。

Disentangling associated genomes.

作者信息

Sloan Daniel B, Bennett Gordon M, Engel Philipp, Williams David, Ochman Howard

机构信息

Department of Ecology and Evolutionary Biology, Yale University, New Haven, Connecticut, USA.

出版信息

Methods Enzymol. 2013;531:445-64. doi: 10.1016/B978-0-12-407863-5.00020-4.

DOI:10.1016/B978-0-12-407863-5.00020-4

PMID:24060132

Abstract

The recovery and assembly of genome sequences from samples containing communities of organisms pose several challenges. Because it is rarely possible to disassociate the resident organisms prior to sequencing, a major obstacle is the assignment of sequences to a single genome that can be fully assembled. This chapter delineates many of the decisions, methodologies, and approaches that can lead to the generation of complete or nearly complete microbial genome sequences from heterogeneous samples-that is, the procedures that allow us to turn metagenomes into genomes.

摘要

从含有生物群落的样本中恢复和组装基因组序列面临着诸多挑战。由于在测序之前很少能够将其中的生物分离，一个主要障碍是将序列分配到可以完全组装的单个基因组上。本章阐述了许多决策、方法和途径，这些能够从异质样本中生成完整或近乎完整的微生物基因组序列，也就是那些能让我们将宏基因组转化为基因组的程序。