Yasuno K, Nishiyama S, Kobayashi R, Yoshimura H, Takahashi K, Omachi T, Kamiie J, Shirota K
Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Department of Veterinary Pathology, Faculty of Veterinary Medicine, Nippon Veterinary and Life Science University, Tokyo, Japan.
J Comp Pathol. 2014 Jan;150(1):18-26. doi: 10.1016/j.jcpa.2013.07.004. Epub 2013 Sep 21.
Non-keratinocyte cells with clear or vacuolated cytoplasm are frequently observed in the epidermis of canine nipples. Most of these cells express cytokeratin (CK) CAM5.2, a marker of luminal epithelial cells. The morphological and immunohistochemical characteristics of these clear cells were investigated. Nipple tissue from 36 dogs of both sexes was collected and labelled immunohistochemically for CAM5.2, CK7, CK14, CK18, CK20, α-smooth muscle actin, p63, melan-A, E-cadherin, epidermal growth factor receptor and oestrogen receptor (OR). The intra-epidermal CAM5.2(+) clear cells were present singly or as small clusters, mostly within the basal layer, in 22 dogs (61%). These cells also expressed CK7, CK18, E-cadherin and OR. Electron microscopy revealed that some of these cells had surface microvilli. Multifocal proliferative lesions consisting of these cells were observed in the nipples of four dogs. In these lesions, proliferating cells formed bilayered tubules with CAM5.2(+) inner and CK14/p63(+) outer cells. This is the first report describing intra-epidermal CAM5.2(+) clear cells, distinct from melanocytes and Merkel cells in dog nipples. These cells might arise from the luminal epithelium of the papillary duct.
在犬乳头表皮中经常观察到细胞质清晰或呈空泡状的非角质形成细胞。这些细胞中的大多数表达细胞角蛋白（CK）CAM5.2，这是一种管腔上皮细胞的标志物。对这些透明细胞的形态学和免疫组织化学特征进行了研究。收集了36只不同性别的犬的乳头组织，并进行免疫组织化学标记，检测CAM5.2、CK7、CK14、CK18、CK20、α-平滑肌肌动蛋白、p63、黑素A、E-钙黏蛋白、表皮生长因子受体和雌激素受体（OR）。在22只犬（61%）中，表皮内CAM5.2阳性透明细胞单独或成小簇存在，大多位于基底层内。这些细胞还表达CK7、CK18、E-钙黏蛋白和OR。电子显微镜显示其中一些细胞有表面微绒毛。在4只犬的乳头中观察到由这些细胞组成的多灶性增殖性病变。在这些病变中，增殖细胞形成双层小管，内层细胞CAM5.2阳性，外层细胞CK14/p63阳性。这是首次报道犬乳头表皮内CAM5.2阳性透明细胞，不同于黑素细胞和默克尔细胞。这些细胞可能起源于乳头管的管腔上皮。
