Chen Yumei, Lin Haitao, Yue Xinxia, Lai Enping, Huang Jiwei, Zhao Ziyu
School of Guangxi Key Laboratory of Sugar Resources of Green Processing, School of Biological and Chemical Engineering, Guangxi University of Science and Technology, Liuzhou 545006, China.
School of Engineering Research Center for Knitting Technology, Ministry of Education, Jiangnan University, Wuxi 214122, China.
Materials (Basel). 2024 Jun 18;17(12):2986. doi: 10.3390/ma17122986.
The application prospects of composite sponges with antibacterial and drug-carrying functions in the field of medical tissue engineering are extensive. A solution of cassava silk fibroin (CSF) was prepared with Ca(NO) as a solvent, which was then combined with chitosan (CS) to create a sponge-porous material by freeze-drying. The CSF-CS composite sponge with a mesh structure was successfully fabricated through hydrogen bonding. Scanning electron microscopy (SEM), Fourier transform infrared absorption (FTIR) and X-ray diffraction (XRD) were employed to investigate the appearance and structure of the cassava silk's fibroin materials, specifically examining the impact of different mass percentages of CS on the sponge's structure. The swelling rate and mechanical properties of the CSF-CS sponge were analyzed, along with its antibacterial properties. Furthermore, by incorporating ibuprofen as a model drug into these loaded sponges, their potential efficacy as efficient drug delivery systems was demonstrated. The results indicate that the CSF-CS sponge possesses a three-dimensional porous structure with over 70% porosity and an expansion rate exceeding 400% while also exhibiting good resistance against pressure. Moreover, it exhibits excellent drug-carrying ability and exerts significant bacteriostatic effects on Escherichia coli. Overall, these findings support considering the CSF-CS composite sponge as a viable candidate for use in drug delivery systems or wound dressings.
具有抗菌和载药功能的复合海绵在医用组织工程领域具有广泛的应用前景。以硝酸钙（Ca(NO)）为溶剂制备木薯丝素蛋白（CSF）溶液，然后将其与壳聚糖（CS）混合，通过冷冻干燥制备海绵状多孔材料。通过氢键成功制备了具有网状结构的CSF-CS复合海绵。采用扫描电子显微镜（SEM）、傅里叶变换红外吸收光谱（FTIR）和X射线衍射（XRD）研究木薯丝素蛋白材料的外观和结构，具体考察不同质量百分比的CS对海绵结构的影响。分析了CSF-CS海绵的溶胀率、力学性能及其抗菌性能。此外，通过将布洛芬作为模型药物掺入这些载药海绵中，证明了它们作为高效药物递送系统的潜在功效。结果表明，CSF-CS海绵具有三维多孔结构，孔隙率超过70%，膨胀率超过400%，同时还具有良好的抗压性。此外，它具有优异的载药能力，对大肠杆菌具有显著的抑菌作用。总体而言，这些研究结果支持将CSF-CS复合海绵视为药物递送系统或伤口敷料的可行候选材料。
