Department of Natural Products, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
Department of Pharmaceutical Technology Biotechnology, National Institute of Pharmaceutical Education and Research, Sector-67, S.A.S. Nagar, 160062 Punjab, India.
Fitoterapia. 2013 Dec;91:290-297. doi: 10.1016/j.fitote.2013.09.008. Epub 2013 Sep 21.
The roots of Potentilla fulgens have been used for a long time as a folk remedy for many ailments without having information on its pharmacological action. Of the various extracts prepared by partitioning of the methanol extract, the ethyl acetate fraction was found to possess better antioxidant and cytotoxic activities. The degree of reduction in cloning efficiencies of MCF-7 cell lines was more with ethyl acetate than with hexane fraction of the root-extract. Hence, this fraction was further purified and nine compounds, including two new ursane type triterpenoids Fulgic acid A (4) and Fulgic acid B (5), were identified and characterized. Other compounds were identified as ursolic acid, euscaphic acid, corosolic acid, epicatechin, catechin, p-hydroxybenzaldehyde and gallic acid. Chemical structures were elucidated by spectroscopic methods, especially ESIHRMS and 2D NMR techniques. The new compounds showed good antioxidant activity and therefore this plant can be a source of natural antioxidants.
金露梅的根在很长一段时间内一直被用作民间药物，用于治疗许多疾病，但没有关于其药理作用的信息。从甲醇提取物的分配制备的各种提取物中，发现乙酸乙酯部分具有更好的抗氧化和细胞毒性活性。与正己烷部分相比，乙酸乙酯部分更能降低 MCF-7 细胞系的克隆效率。因此，该部分进一步纯化，并鉴定和表征了包括两种新的五环三萜酸 Fulgic 酸 A（4）和 Fulgic 酸 B（5）在内的 9 种化合物。其他化合物被鉴定为熊果酸、羽扇豆酸、齐墩果酸、表儿茶素、儿茶素、对羟基苯甲醛和没食子酸。通过光谱方法，特别是 ESIHRMS 和 2D NMR 技术阐明了化学结构。新化合物表现出良好的抗氧化活性，因此这种植物可以作为天然抗氧化剂的来源。
