Demirkòran Nizamettin, Ekinci Ergun
Acta Chim Slov. 2012 Jun;59(2):302-6.
An electrochemical biosensor was developed by using the sol-gel coating solution. The modified platinum electrode used in the study was constructed by immobilization of glucose oxidase under a layer of sol-gel film. The coating solution was prepared by using GLYMO, and MTEOS. Electrochemical measurements were carried out amperometrically by determining hydrogen peroxide produced by the enzymatic reaction between glucose and glucose oxidase. It was observed that the amperometric response of the enzyme electrode was linear for glucose concentrations in the range from 2 to10 mM. The response time of the biosensor to reach a steady-state current value was approximately 80 s. The glucose selectivity of the biosensor was determined in the presence of some interfering substances, such as lactose, sucrose, urea, uric acid, oxalic acid, and ascorbic acid.It was observed that the interfering molecules did not cause any observable signal. The stability of the sensor was investigated, and it was found hat the initial sensor response decreased approximately 44% over a period of 30 days of storage in dry state at 4 °C.
通过使用溶胶 - 凝胶涂层溶液开发了一种电化学生物传感器。该研究中使用的修饰铂电极是通过在一层溶胶 - 凝胶膜下固定葡萄糖氧化酶构建的。涂层溶液是通过使用γ-缩水甘油醚氧丙基三甲氧基硅烷（GLYMO）和甲基三乙氧基硅烷（MTEOS）制备的。通过测定葡萄糖与葡萄糖氧化酶之间的酶促反应产生的过氧化氢，以安培法进行电化学测量。观察到酶电极的安培响应对于2至10 mM范围内的葡萄糖浓度呈线性。生物传感器达到稳态电流值的响应时间约为80秒。在存在一些干扰物质（如乳糖、蔗糖、尿素、尿酸、草酸和抗坏血酸）的情况下测定了生物传感器的葡萄糖选择性。观察到干扰分子没有引起任何可观察到的信号。研究了传感器的稳定性，发现在4°C干燥状态下储存30天期间，传感器的初始响应下降了约44%。
