The Donnelly Centre, University of Toronto, , 160 College St., Toronto, Ontario, Canada , M5S 3E1.
Philos Trans R Soc Lond B Biol Sci. 2013 Sep 23;368(1629):20130118. doi: 10.1098/rstb.2013.0118. Print 2013.
The budding yeast Saccharomyces cerevisiae has been used extensively for the study of cell polarity, owing to both its experimental tractability and the high conservation of cell polarity and other basic biological processes among eukaryotes. The budding yeast has also served as a pioneer model organism for virtually all genome-scale approaches, including functional genomics, which aims to define gene function and biological pathways systematically through the analysis of high-throughput experimental data. Here, we outline the contributions of functional genomics and high-throughput methodologies to the study of cell polarity in the budding yeast. We integrate data from published genetic screens that use a variety of functional genomics approaches to query different aspects of polarity. Our integrated dataset is enriched for polarity processes, as well as some processes that are not intrinsically linked to cell polarity, and may provide new areas for future study.
出芽酵母酿酒酵母由于其实验可操作性以及细胞极性和真核生物中其他基本生物过程的高度保守性，被广泛用于细胞极性的研究。出芽酵母还作为几乎所有基因组规模方法的先驱模式生物，包括功能基因组学，其目的是通过分析高通量实验数据系统地定义基因功能和生物途径。在这里，我们概述了功能基因组学和高通量方法对出芽酵母细胞极性研究的贡献。我们整合了使用各种功能基因组学方法来查询不同方面极性的已发表遗传筛选数据。我们的综合数据集富含极性过程，以及一些与细胞极性没有内在联系的过程，并且可能为未来的研究提供新的领域。
