Department of Chemistry, University of Liverpool , Liverpool L69 7ZD, United Kingdom.
ACS Nano. 2013 Oct 22;7(10):8881-90. doi: 10.1021/nn403350v. Epub 2013 Sep 26.
Differential centrifugal sedimentation (DCS) has been applied to accurately size ligand-protected gold hydrosols in the 10 to 50 nm range. A simple protocol is presented to correct for particle density variations due to the presence of the ligand shell, which is formed here by either polyethylene glycol-substituted alkane thiols (PEG-alkane thiols) of different chain length or oligopeptides. The method gives reliable data for all particle sizes investigated and lends itself to rapid routine sizing of nanoparticles. Unlike TEM, DCS is highly sensitive to small changes in the thickness of the organic ligand shell and can be applied to monitor shell thickness variations of as little as 0.1 nm on particles of a given core size.
差速离心沉降法（DCS）已被应用于准确测量 10 至 50nm 范围内配体保护的金纳米水溶胶的粒径。本文提出了一种简单的方案来校正由于配体壳的存在而导致的颗粒密度变化，这里的配体壳是由不同链长的聚乙二醇取代烷硫醇（PEG-烷硫醇）或寡肽形成的。该方法可对所有研究的颗粒尺寸提供可靠的数据，适用于快速常规纳米颗粒的尺寸测量。与 TEM 不同，DCS 对有机配体壳厚度的微小变化非常敏感，可用于监测给定核尺寸颗粒上壳厚度变化小至 0.1nm。
