基于单颗粒电感耦合等离子体质谱（spICP-SFMS）和多角度激光散射联用四极杆飞行时间质谱（EAF4-MALS）分析方法对合成糖聚合物共轭金纳米棒的表征

Characterization of Gold Nanorods Conjugated with Synthetic Glycopolymers Using an Analytical Approach Based on spICP-SFMS and EAF4-MALS.

作者信息

Velimirovic Milica, Pancaro Alessia, Mildner Robert, Georgiou Panagiotis G, Tirez Kristof, Nelissen Inge, Johann Christoph, Gibson Matthew I, Vanhaecke Frank

机构信息

Department of Chemistry, Atomic & Mass Spectrometry-A&MS Research Group, Campus Sterre, Ghent University, Krijgslaan 281-S12, 9000 Ghent, Belgium.

Flemish Institute for Technological Research (VITO), Boeretang 200, 2400 Mol, Belgium.

出版信息

Nanomaterials (Basel). 2021 Oct 15;11(10):2720. doi: 10.3390/nano11102720.

A new comprehensive analytical approach based on single-particle inductively coupled plasma-sector field mass spectrometry (spICP-SFMS) and electrical asymmetric-flow field-flow-fractionation combined with multi-angle light scattering detection (EAF4-MALS) has been examined for the characterization of galactosamine-terminated poly(N-hydroxyethyl acrylamide)-coated gold nanorods (GNRs) in two different degrees of polymerization (DP) by tuning the feed ratio (short: DP 35; long: DP 60). spICP-SFMS provided information on the particle number concentration, size and size distribution of the GNRs, and was found to be useful as an orthogonal method for fast characterization of GNRs. Glycoconjugated GNRs were separated and characterized via EAF4-MALS in terms of their size and charge and compared to the bare GNRs. In contrast to spICP-SFMS, EAF4-MALS was also able of providing an estimate of the thickness of the glycopolymer coating on the GNRs surface.

一种基于单颗粒电感耦合等离子体质谱仪-扇形磁场质谱仪（spICP-SFMS）以及结合多角度光散射检测的不对称流场流分馏仪（EAF4-MALS）的新型综合分析方法，已被用于表征通过调节进料比制备的两种不同聚合度（DP）的半乳糖胺封端的聚（N-羟乙基丙烯酰胺）包覆金纳米棒（GNR）（短链：DP 35；长链：DP 60）。spICP-SFMS提供了有关GNR的颗粒数浓度、尺寸和尺寸分布的信息，并被发现是一种用于快速表征GNR的正交方法。糖缀合GNR通过EAF4-MALS根据其尺寸和电荷进行分离和表征，并与裸GNR进行比较。与spICP-SFMS不同，EAF4-MALS还能够估计GNR表面糖聚合物涂层的厚度。