Whitlock Patrick W, Seyler Thorsten M, Northam Casey N, Smith Thomas L, Poehling Gary G, Koman L Andrew, Van Dyke Mark E
Department of Orthopaedic Surgery, Wake Forest University Health Sciences, Medical Center Boulevard, Winston-Salem, NC 27157-1070.
J Surg Orthop Adv. 2013 Fall;22(3):224-32. doi: 10.3113/jsoa.2013.0224.
Naturally derived tendon scaffolds have the potential to improve the treatment of flexor tendon injuries. Seeded and unseeded tendon scaffolds were maintained in the presence or absence of physiologic strain for 7 days. After 7 days, the tensile properties and associated messenger RNA expression were compared. Seeded scaffolds maintained in the absence of strain had significantly lower tensile properties than unseeded tendons and fresh-frozen tendons. The loss of tensile properties was associated with elevated matrix metalloproteinase-2 and collagen III expression. Tensile properties of seeded scaffolds maintained in the presence of strain for 7 days after seeding did not differ from those of fresh-frozen tendons. This study demonstrates that the tensile properties of seeded, naturally derived tendon scaffolds will degrade rapidly in the absence of cyclic strain. Seeded scaffolds used for tendon reconstruction should be maintained under cyclic strain to maintain essential tensile properties.
天然来源的肌腱支架具有改善屈肌腱损伤治疗效果的潜力。接种和未接种的肌腱支架在有或无生理应变的情况下维持7天。7天后，比较拉伸性能和相关信使核糖核酸表达。在无应变情况下维持的接种支架的拉伸性能明显低于未接种肌腱和新鲜冷冻肌腱。拉伸性能的丧失与基质金属蛋白酶-2和胶原蛋白III表达升高有关。接种后在有应变情况下维持7天的接种支架的拉伸性能与新鲜冷冻肌腱的拉伸性能没有差异。本研究表明，在没有循环应变的情况下，接种的天然来源肌腱支架的拉伸性能将迅速降解。用于肌腱重建的接种支架应在循环应变下维持以保持基本的拉伸性能。
