N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky prosp., 47, Moscow, 119991, Russian Federation.
Glycoconj J. 2014 Jan;31(1):7-12. doi: 10.1007/s10719-013-9497-3. Epub 2013 Sep 25.
Galectins are multifunctional effectors, for example acting as regulators of cell growth via protein-glycan interactions. The observation of capacity to kill bacteria for two tandem-repeat-type galectins, which target histo-blood epitopes toward this end (Stowell et al. Nat. Med. 16:295-301, 2010), prompted us to establish an array with bacterial polysaccharides. We addressed the question whether sugar determinants other than β-galactosides may be docking sites, using human galectins-4, -8, and -9. Positive controls with histo-blood group ABH-epitopes and the E. coli 086 polysaccharide ascertained the suitability of the set-up. Significant signal generation, depending on type of galectin and polysacchride, was obtained. Presence of cognate β-galactoside-related epitopes within a polysaccharide chain or its branch will not automatically establish binding properties, and structural constellations lacking galactosides, like rhamnan, were found to be active. These data establish the array as valuable screening tool, giving direction to further functional and structural studies.
半乳糖凝集素是多功能效应物，例如通过蛋白-糖相互作用来调节细胞生长。我们观察到两种串联重复型半乳糖凝集素具有杀菌能力，这些凝集素将组织血液表位作为靶标（Stowell 等人，《自然医学》16:295-301，2010），这促使我们建立了一个含有细菌多糖的阵列。我们使用人源半乳糖凝集素-4、-8 和 -9 来研究除了β-半乳糖苷之外的糖决定簇是否可能是结合位点的问题。带有组织血液组 ABH 表位的阳性对照和大肠杆菌 086 多糖证实了该设置的适用性。根据半乳糖凝集素和多糖的类型，获得了显著的信号生成。多糖链或其支链内存在同源β-半乳糖苷相关表位不会自动建立结合特性，并且发现缺乏半乳糖苷的结构组合，如鼠李聚糖，具有活性。这些数据确立了该阵列作为有价值的筛选工具，为进一步的功能和结构研究提供了方向。
